Packages (Simulation)
![Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/product/LM.jpg)
Reagent Preparation
![Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/reagent/TLMB.jpg)
Image (I)
Image (II)
Certificate
![Certificate](/upload/static/ISO13485.jpg)
![Certificate](/upload/static/ce-1.jpg)
Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA638Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.1-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.033 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 393
US$ 408
US$ 431
US$ 461
US$ 491
US$ 537
US$ 605
Result
For more details, please contact local distributors! US$ 756 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-103 | 91 |
EDTA plasma(n=5) | 92-101 | 97 |
heparin plasma(n=5) | 92-102 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 88-95% | 78-103% | 94-105% | 84-104% |
EDTA plasma(n=5) | 93-103% | 86-103% | 91-99% | 86-103% |
heparin plasma(n=5) | 94-105% | 81-98% | 84-102% | 85-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:VF) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
Magazine | Citations |
Brazilian Journal of Biomoricity | EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN Brjb: source |
Diabetes Research and Clinical Practice | The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance ScienceDirect: S0168822711002956 |
J Physiol Pharmacol. | Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. Pubmed: 24304571 |
Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658 |
Exp Ther Med. | Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome Pubmed:Pmc3991522 |
Eur J Cancer Prev. | Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. Pubmed:25055182 |
Genome | Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases Nrcresearchpress:Source |
PLOS ONE | Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug PubMed: 26262991 |
Alcoholism and Drug Addiction | Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males Science: Article |
Cytokine | Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies PubMed: 26539806 |
Genome | Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications PubMed: 25120107 |
Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234 |
Endocrine, Metabolic & Immune Disorders-Drug Targets | Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. pubmed:28699485 |
Endocrine Connections | Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery Pubmed:30012648 |
Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871 |
Contraception | Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive Pubmed:29665358 |
Cranio - Journal of Craniomandibular Practice | Analysis of synovial fluid visfatin level in temporomandibular joint disorders Pubmed:29385907 |
Nutrire | Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children 10.1186:s41110-018-0060-7 |
DNA and Cell Biology | Transcriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes Pubmed: 30615481 |
Jundishapur Journal of Chronic Disease Care | The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men |
Biomarkers in Heart Failure and Associated Diseases | Adipokines as Biomarkers in Health and Disease |
Journal of Translational Medicine | Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern Pubmed: 31533725 |
clinical neurology and neurosurgery | Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels Pubmed: 32311618 |
The Horizon of Medical Sciences | Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females |
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA638Hu01 | Recombinant Visfatin (VF) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA638Hu21 | OVA Conjugated Visfatin (VF) | Immunogen; SDS-PAGE; WB. |
PAA638Hu01 | Polyclonal Antibody to Visfatin (VF) | WB; IHC |
PAA638Hu08 | Polyclonal Antibody to Visfatin (VF) | WB; IHC; ICC; IP. |
FAA638Hu01 | Anti-Visfatin (VF) Polyclonal Antibody | Flow cytometry. |
MAA638Hu22 | Monoclonal Antibody to Visfatin (VF) | WB |
MAA638Hu27 | Monoclonal Antibody to Visfatin (VF) | WB; IHC; ICC; IP. |
MAA638Hu29 | Monoclonal Antibody to Visfatin (VF) | WB; IHC; ICC; IP. |
MAA638Hu21 | Monoclonal Antibody to Visfatin (VF) | WB |
MAA638Hu23 | Monoclonal Antibody to Visfatin (VF) | WB; IHC; ICC; IP. |
MAA638Hu24 | Monoclonal Antibody to Visfatin (VF) | WB; IHC |
MAA638Hu25 | Monoclonal Antibody to Visfatin (VF) | WB; IHC; ICC; IP. |
MAA638Hu26 | Monoclonal Antibody to Visfatin (VF) | WB; IHC; ICC; IP. |
FAA638Hu04 | Anti-Visfatin (VF) Monoclonal Antibody | Flow cytometry. |
FAA638Hu02 | Anti-Visfatin (VF) Monoclonal Antibody | Flow cytometry. |
FAA638Hu06 | Anti-Visfatin (VF) Monoclonal Antibody | Flow cytometry. |
FAA638Hu08 | Anti-Visfatin (VF) Monoclonal Antibody | Flow cytometry. |
SEA638Hu | ELISA Kit for Visfatin (VF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA638Hu | Mini Samples ELISA Kit for Visfatin (VF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA638Hu | ELISA Kit for Visfatin (VF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA638Hu | Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA638Hu11 | ELISA Kit DIY Materials for Visfatin (VF) | Main materials for "Do It (ELISA Kit) Yourself". |