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Multiplex Assay Kit for Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-103 88
EDTA plasma(n=5) 91-98 94
heparin plasma(n=5) 80-99 80

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 88-97% 98-105% 95-105%
EDTA plasma(n=5) 97-105% 79-102% 92-105% 86-97%
heparin plasma(n=5) 80-103% 93-101% 84-95% 78-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NGB) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Morfovirtual 2014 EFECTO PROTECTOR DEL TRATAMIENTO CON NEUROEPO SOBRE LA GLIA DE LA CORTEZA CEREBRAL EN RATONES TRANSGéNICOS CON LA ENFERMEDAD DE ALZHEIMER. Sld:Source
Critical Care Medicine Expression and Role of Neuroglobin in Rats With Sepsis-Associated Encephalopathy Pubmed:24145836
Asian Journal of Pharmaceutical and Clinical Research RESPONSES OF BRAIN TISSUES AGAINST HYPOXIC CONDITION IN HEMORRHAGIC STROKE PATIENTS: NEUROGLOBIN EXPRESSION IN BRAIN TISSUE AND PLASMA 10.22159/ajpcr.2017.v10i2.15971
Neurobiology of Disease Hypoxia promotes tau hyperphosphorylation with associated neuropathology in vascular dysfunction Pubmed:30010004
Environ Toxicol Pharmacol Neuroglobin Alleviates Arsenic-Induced Neuronal Damage 33545379
Medicine (Baltimore) Plasma S100¦Â and neuron-specific enolase, but not neuroglobin, are associated with early cognitive dysfunction after total arch replacement surgery: A pilot?¡­ 33847649
Muhammadiyah Journal of Geriatric Kadar Neuroglobin dan Sitoglobin dalam Plasma, Cairan Serebro Spinalis, dan Jaringan Otak Pasien Strok Hemoragik
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA606Ra01 Recombinant Neuroglobin (NGB) Positive Control; Immunogen; SDS-PAGE; WB.
PAA606Ra01 Polyclonal Antibody to Neuroglobin (NGB) WB; IHC; ICC; IP.
MAA606Ra21 Monoclonal Antibody to Neuroglobin (NGB) WB; IHC; ICC; IP.
SEA606Ra ELISA Kit for Neuroglobin (NGB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA606Ra Multiplex Assay Kit for Neuroglobin (NGB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.