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Multiplex Assay Kit for Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA548Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 348
US$ 362
US$ 382
US$ 409
US$ 436
US$ 476
US$ 536
Result
For more details, please contact local distributors! US$ 670 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 97-105 | 101 |
EDTA plasma(n=5) | 87-96 | 90 |
heparin plasma(n=5) | 89-97 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 93-104% | 86-99% | 78-94% | 90-97% |
EDTA plasma(n=5) | 90-98% | 96-105% | 90-98% | 81-91% |
heparin plasma(n=5) | 88-97% | 92-99% | 83-103% | 97-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:ICAM1) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Amran et al. Lipids in Health and Disease | Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. BioMed: 1476511X |
Evidence-based Complementary and Alternative Medicine | Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats PubMed: PMC3703719 |
British Journal of Medical and Health Sciences | EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS Researchgate: Source |
Cytokine | Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. Pubmed: 23434274 |
BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192 |
Journal of Veterinary Internal Medicine | Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis Pubmed: 23875771 |
Arch Dis Child | PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns Bmj:Source |
Arch Dis Child? | PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome Bmj:Source |
Arch Dis Child | PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography Bmj:Source |
J Thorac Dis.? | Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension Pubmed:Pmc4015009 |
Journal of Immunotoxicology | Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice Pubmed:24873678 |
Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835 |
Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits Pubmed:25111247 |
Naunyn-Schmiedeberg's Archives of Pharmacology | Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits Pubmed:25172523 |
Nutr Res. | Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits Pubmed:25282128 |
Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns Pubmed:25358349 |
Neural Regen Res.? | Inflammatory response and neuronal necrosis in rats with cerebral ischemia Pubmed:25422636 |
Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880 |
CNS Neuroscience & Therapeutics | Peroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats Pubmed:25611692 |
Alexandria Journal of Medicine | Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats Sciencedirect:S2090506814000268 |
Canadian Journal of Physiology and Pharmacology | Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism Doi: Abs |
J Cell Biochem | Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms PubMed: 26177712 |
Neurotoxicology | Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats PubMed: 25845300 |
Alexandria Journal of Medicine | Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Science: Article |
Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880 |
Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140 |
Experimental Eye Research | The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoaCavia (Guinea pig )lation in pigmented rabbits Pubmed:27296072 |
Chinese Chemical Letters | Screening the anti-gout traditional herbs from TCM using an in vitro method science:S1001841716301450 |
Am J Physiol Lung Cell Mol Physiol | Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis Pubmed:26993522 |
Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655 |
Comparative Clinical Pathology | Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets article:10.1007 |
Journal of Cellular Biochemistry | Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms pubmed:26177712 |
BMC Vet Res. | Blood markers of fibrinolysis and endothelial activation in canine babesiosis. pubmed:28363279 |
Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie | Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion pubmed:28273631 |
Pakistan journal of pharmaceutical sciences | Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. pubmed:29084680 |
Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422 |
Molecular Medicine Reports | Age‑related differences in serum MFG‑E8, TGF‑β1 and correlation to the severity of atherosclerosis determined by ultrasound. pubmed:29152651 |
Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573 |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858 |
International Journal of Clinical and Experimental Pathology | Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study ISSN:1936-2625/IJCEP0055881 |
Oncotarget | Global gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO model pubmed:29088811 |
Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275 |
Journal of ophthalmology | Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy pubmed:28761761 |
The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice Pubmed:29929978 |
Life Sciences | Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation Pubmed:29499280 |
International Journal of Molecular Medicine | Aspirin modulates the inflammatory response in a thrombus‑stimulated LMVEC model Pubmed:29568915 |
Clinical And Experimental Pharmacology And Physiology | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway Pubmed:29266336 |
Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010 |
Bioscience Reports | N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. Doi: 10.1042/BSR20180858 |
Prostaglandins & Other Lipid Mediators | Detoxification and activating blood circulation decoction reduces restenosis involving the TLR4/NF-κB pathway after balloon injury Pubmed: 30453089 |
Biochemical and Biophysical Research Communications | The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion Pubmed: 30558792 |
Nutrition & Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women Pubmed: 30485835 |
Cytokine | Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls Pubmed: 30716657 |
European Journal of Pharmacology | The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in … Pubmed: 30826323 |
International journal of molecular sciences | Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial Dysfunction Pubmed: 31261711 |
Experimental cell research | Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts Pubmed: 31233740 |
international journal of molecular sciences | Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells Pubmed: 31671764 |
Archives of Insect Biochemistry and Physiology | Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia Pubmed: 31774317 |
BMC Endocrine Disorders | The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561 |
Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827 |
Journal of Applied Microbiology | The effects of dietary sodium butyrate supplementation on the growth performance, carcass traits, and intestinal microbiota of growing‐finishing pigs Pubmed: 32048746 |
LIFE SCIENCES | Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress Pubmed: 33227274 |
Metab Brain Dis | Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats Pubmed: 33170419 |
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice Pubmed: 33065235 |
BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats Pubmed: 33028437 |
MOLECULAR AND CELLULAR BIOCHEMISTRY | MicroRNA-217 ameliorates inflammatory damage of endothelial cells induced by oxidized LDL by targeting EGR1 Pubmed: 32737769 |
OBESITY SURGERY | The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized … Pubmed: 32535786 |
Inflammation | Tetramethylpyrazine Inhibits Platelet Adhesion and Inflammatory Response in Vascular Endothelial Cells by Inhibiting P38 MAPK and NF-κB Signaling Pathways |
J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555 |
Molecules | Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion 33430011 |
Antioxidants (Basel) | Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model 33562069 |
J Vet Intern Med | Association of markers of endothelial activation and dysfunction with occurrence and outcome of pulmonary hemorrhage in dogs with leptospirosis 34076314 |
PLoS One | A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … 34492054 |
Sci. Pharm | Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism |
Journal of the South African Veterinary Association | Circulating markers of endothelial activation in canine parvoviral enteritis |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA548Mu02 | Recombinant Intercellular Adhesion Molecule 1 (ICAM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA548Mu01 | Recombinant Intercellular Adhesion Molecule 1 (ICAM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA548Mu01 | Polyclonal Antibody to Intercellular Adhesion Molecule 1 (ICAM1) | WB; IHC; ICC; IP. |
PAA548Mu02 | Polyclonal Antibody to Intercellular Adhesion Molecule 1 (ICAM1) | WB; IHC; ICC; IP. |
SEA548Mu | ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA548Mu | Mini Samples ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA548Mu | CLIA Kit for Intercellular Adhesion Molecule 1 (ICAM1) | Chemiluminescent immunoassay for Antigen Detection. |
LMA548Mu | Multiplex Assay Kit for Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |