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![Multiplex Assay Kit for Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/product/LM.jpg)
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Multiplex Assay Kit for Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay)
Myelin A1 protein; Myelin membrane encephalitogenic protein
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA539Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids.
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
Result
For more details, please contact local distributors! US$ 821 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-93 | 83 |
EDTA plasma(n=5) | 93-102 | 99 |
heparin plasma(n=5) | 80-96 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 78-95% | 85-93% | 92-101% | 99-105% |
EDTA plasma(n=5) | 81-93% | 97-105% | 94-105% | 92-101% |
heparin plasma(n=5) | 80-92% | 96-103% | 92-104% | 85-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:MBP) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source |
Journal of Occupational Health | Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain Pubmed: 23183024 |
Pak J Med Sci | Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke cases PubMed: 26648996 |
Experimental and Toxicologic Pathology | Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus) Pubmed:27165445 |
Acta Angiologica | Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy AA.2016.0008 |
Stem Cells. | Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. pubmed:27573615 |
Environmental Science and Pollution Research | Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures. pubmed:27696165 |
Acta Angiol | Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy AA.2016.0008 |
PLoS Neglected Tropical Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis pubmed:28489854 |
Pesticide Biochemistry and Physiology | Acrolein-mediated neurotoxicity in growing Wistar male rats Pubmed:30033014 |
International Journal of Livestock Research | Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies 10.5455:ijlr.20170811080455 |
Microchimica Acta | Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a review Pubmed: 31760523 |
The role of light desynchronosis in the development of stress-induced aging | |
Adv Med Sci | Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring 34091432 |
biomolecules | Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡ |
Talanta | Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in … Pubmed:35202838 |
Catalog No. | Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species | Applications (RESEARCH USE ONLY!) |
RPA539Rb01 | Recombinant Myelin Basic Protein (MBP) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA539Rb51 | Polyclonal Antibody to Myelin Basic Protein (MBP) | WB; IHC; ICC; IP. |
MAA539Rb21 | Monoclonal Antibody to Myelin Basic Protein (MBP) | WB; IHC; ICC; IP. |
SEA539Rb | ELISA Kit for Myelin Basic Protein (MBP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA539Rb | Multiplex Assay Kit for Myelin Basic Protein (MBP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |