≡

Multiplex Assay Kit for Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay)

FnDP; Fibrin Split Products; Fibrin Degradation Product

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-99 88
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 85-99 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-98% 92-101% 78-104% 84-98%
EDTA plasma(n=5) 88-95% 93-101% 96-104% 80-90%
heparin plasma(n=5) 86-101% 83-92% 84-101% 90-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FDP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Am J Physiol Lung Cell Mol Physiol The role of the E2F1 transcription factor in the innate immune response to systemic LPS Physiology: Source
Bone Marrow Transplant. Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleen Pubmed:25243628
Nephrology Dialysis Transplantation The efficacy of recombinant human soluble thrombomodulin for the treatment of shiga toxin associated hemolytic uremic syndrome model mice Pubmed:25694534
Gastroenterology Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9–Mediated Cytokine Release From Myeloid Cells Sciencedirect:S0016508514014851
Gastroenterology Inhibition of Plasmin Protects Against Colitis in Mice by PubMed: 25490065
Haematologica Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain Pubmed:29903766
Bone Temporary inhibition of the plasminogen activator inhibits periosteal chondrogenesis and promotes periosteal osteogenesis during appendicular bone fracture … 10.1016:j.bone.2018.04.016
Thrombosis andHaemostasis Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome Pubmed: 29202212
Critical care Co-administration of iloprost and eptifibatide in septic shock (CO-ILEPSS)—a randomised, controlled, double-blind investigator-initiated trial investigating … Pubmed: 31488213
Journal of Thrombosis and Haemostasis Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats Pubmed: 31755229
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
CEA530Rb ELISA Kit for Fibrinogen Degradation Product (FDP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA530Rb Multiplex Assay Kit for Fibrinogen Degradation Product (FDP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.