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Multiplex Assay Kit for Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay)
CALCA; CALC1; CGRP-I; CGRP1; KC; CCP; PDN-21; Katacalcin; Calcitonin-Related Polypeptide Alpha; Calcitonin carboxyl-terminal peptide
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA472Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 349
US$ 363
US$ 383
US$ 410
US$ 437
US$ 477
US$ 538
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 89-96 | 92 |
EDTA plasma(n=5) | 79-91 | 86 |
heparin plasma(n=5) | 87-94 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 94-101% | 83-90% | 98-105% | 80-92% |
EDTA plasma(n=5) | 79-101% | 80-95% | 83-101% | 80-103% |
heparin plasma(n=5) | 89-103% | 84-95% | 97-105% | 96-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:CT) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
indian Journal of Experimental Biology | Effect of consumption of fatty acids, calcium, Vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats Niscair: 13737 |
Toxicol Ind Health | The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709 |
The Journal of Clinical Endocrinology & Metabolism | Interleukin-2 and Lanreotide in the Treatment of Medullary Thyroid Cancer: In Vitro and In Vivo Studies Pubmed: 23884781 |
Academic Journal | Effect of whole body vibration on healthy rat plasma parameters Ebscohost: Source |
Indian Journal of Experimental Biology | Effect of consumption of fatty acids, nutrients and regular physical activity on bone mechanical properties in rats Pubmed:22439438 |
International journal of cardiology | Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535 |
7 | Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. pubmed:28218908 |
International Journal of Advanced Research in Biological Sciences | Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 10.22192/ijarbs.2017.04.04.018 |
cell proliferation | p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway Pubmed: 32215984 |
Nature | Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia Pubmed: 33149301 |
Clin Chim Acta | Copeptin in fluid disorders and stress Pubmed:35143773 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA472Hu01 | Recombinant Calcitonin (CT) | Positive Control; Immunogen; SDS-PAGE; WB. |
SPA472Hu01 | Synthetic Calcitonin (CT) | Immunogen; Blocking Peptide. |
CPA472Hu21 | OVA Conjugated Calcitonin (CT) | Immunogen; SDS-PAGE; WB. |
CPA472Hu11 | BSA Conjugated Calcitonin (CT) | Immunogen; SDS-PAGE; WB. |
PAA472Hu02 | Polyclonal Antibody to Calcitonin (CT) | WB; IHC |
PAA472Hu01 | Polyclonal Antibody to Calcitonin (CT) | WB; IHC; ICC; IP. |
LAA472Hu71 | Biotin-Linked Polyclonal Antibody to Calcitonin (CT) | WB; IHC; ICC. |
MAA472Hu22 | Monoclonal Antibody to Calcitonin (CT) | WB; IHC; ICC; IP. |
CEA472Hu | ELISA Kit for Calcitonin (CT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA472Hu | Multiplex Assay Kit for Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA472Hu11 | ELISA Kit DIY Materials for Calcitonin (CT) | Main materials for "Do It (ELISA Kit) Yourself". |