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![Multiplex Assay Kit for Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/product/LM.jpg)
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![Multiplex Assay Kit for Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/reagent/TLMB.jpg)
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Multiplex Assay Kit for Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay)
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA471Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
Result
For more details, please contact local distributors! US$ 821 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 79-90 | 87 |
EDTA plasma(n=5) | 80-102 | 91 |
heparin plasma(n=5) | 83-101 | 87 |
sodium citrate plasma(n=5) | 81-98 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-99% | 92-102% | 89-101% | 88-97% |
EDTA plasma(n=5) | 80-101% | 79-104% | 80-93% | 85-93% |
heparin plasma(n=5) | 89-104% | 83-94% | 88-96% | 85-102% |
sodium citrate plasma(n=5) | 90-98% | 87-97% | 96-103% | 85-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:OC) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Acta Biomaterialia | Surface engineering of titanium with potassium hydroxide and its effects on the growth behavior of mesenchymal stem cells PubMed: 19963080 |
Annals of RSCB | Biochemical study of the influence of ad type biophytomodulators in healing of bone defects in rats. Analelesnbc: source |
Asian-Australasian Association of Animal Societies | Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter? Ajas: source |
Nutrition | Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity ScienceDirect: S0899900709004717 |
Experimental Biology and Medicine | Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured mice Rsm: source |
Archives of Oral Biology | Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats ScienceDirect: S0003996912001021 |
22 | Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces ScienceDirect: S0142961212000865 |
Life Sciences | Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes PubMed: 23357248 |
Romanian Journal of Morphology and Embryology | Serum changes induced by intramedullar experimental administration of bisphosphonates PubMed: 21424089 |
International Journal of Cardiology | Valvular osteoclasts in calcification and aortic valve stenosis severity PubMed: 23452891 |
Journal of Physiology and Pharmacology | Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone PubMed: 23211309 |
Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source |
Chinese Medicine | Puerarin promotes osteogenesis and inhibits adipogenesis in vitro PubMed: PMC3765709 |
Nephrology Dialysis Transplantation | Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells PubMed: PMC3611891 |
J Anim Physiol Anim Nutr (Berl). | Morphological changes of the cartilage and bone in newborn piglets evoked by experimentally induced glucocorticoid excess during pregnancy Pubmed: 22716040 |
Journal of Cranio-Maxillofacial Surgery | The use of Tri-Calcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study Pubmed: 24485270 |
Nutr Hosp. | Effect of the “protein diet” and bone tissue. Pubmed: 24483972 |
International Journal of Cardiology | Valvular osteoclasts in calcification and aortic valve stenosis severity. Pubmed: 23452891 |
Food Science & Nutrition | Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 Onlinelibrary: fsn3.109 |
Nutricion Hospitalaria | Effect of the “protein diet” and bone tissue Aulamedica: Source |
Tissue Eng Part A. | Toward the Development and Characterization of an Easy Handling Sheet-Like Biohybrid Bone Substitute Pubmed:25761235 |
Basic & Clinical Pharmacology & Toxicology | Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source |
Food Sci Nutr. | Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 Pubmed:Pmc4221832 |
Journal of Cranio-Maxillofacial Surgery | The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study Pubmed:24485270 |
Naunyn-Schmiedeberg's Archives of Pharmacology | Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats Pubmed:25038619 |
FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352 |
American Diabetes Association | Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopenia Pubmed:25633418 |
International Journal of Nanomedicine | Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation Pubmed:25678785 |
Medical Journal fo the Islamic Republic of Iran | Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat models Pubmed:25679008 |
BioMed Research International | Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathway Pubmed:25834823 |
Advances in Medical Sciences | The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in rats PubMed: 26569440 |
journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb |
Bioscience Trends | Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cells PubMed: 26355224 |
J Nutr Biochem | Body fat loss induced by calcium in co-supplementation with conjugated linoleic acid is associated with increased expression of bone formation genes in adult mice PubMed: 26454511 |
Int J Mol Med | Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway PubMed: 26677102 |
Experimental And Therapeutic Medicine | Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway Pubmed:27347050 |
International Journal of Life Sciences Research | Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats profile:303311214 |
Journal of Alloys and Compounds | Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implants science:S0045653516302016 |
Bone | The effects of strength training and raloxifene on bone health in aging ovariectomized rats Pubmed:26812611 |
Biological Trace Element Research | Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signalingand Insulin Sensitivity in Ovariectomized Rats. Pubmed:26876375 |
ANNALS OF ANIMAL SCIENCE | Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87 |
Annals of Animal Science | Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87 |
J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967 |
Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats : | |
Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561 |
international journal of molecular sciences | Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. pubmed:28272338 |
Applied Surface Science | The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorption S0169433216326861 |
Nutrition. | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. pubmed:27908549 |
Chemico-Biological Interactions | Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway pubmed:27387537 |
Biomedicine & Pharmacotherapy | Fast and long acting neoflavonoids dalbergin isolated from Dalbergia sissoo heartwood is osteoprotective in ovariectomized model of osteoporosis: Osteoprotective … S0753332216308484 |
Experimental and Therapeutic Medicine | Serum N-terminal telopeptide of type I collagen as an early marker of fracture nonunion in rabbits. pubmed:28105092 |
Institute of Pharmacology | Effects of long-term administration of pantoprazole on bone mineral density in young male rats. pubmed:27552060 |
Indian Journal of Orthopaedics | N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits. pubmed:27512225 |
Biological Trace Element Research | Micromolar Levels of Sodium Fluoride Promote Osteoblast Differentiation Through Runx2 Signaling pubmed:28083749 |
J Trace Elem Med Biol | Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats. pubmed:27908407 |
Mater Sci Eng C Mater Biol Appl. | Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation. pubmed:27987727 |
Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752 |
Biological Trace Element Research | Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007 |
Revista Brasileira de Ciência Avícola | Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285 |
Pharmacological Reports | Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopenia pubmed:29031689 |
Journal of Bone and Mineral Metabolism | MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967 |
Oncotarget | A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice. pubmed:28060768 |
Journal of Inorganic Biochemistry | Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953 |
Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995 |
Asian Journal of Biomedical and Pharmaceutical Sciences | A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ò-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X |
Journal of Materials Chemistry B | Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances 10.1039/C7TB02620J |
Journal of Cellular Physiology | Osteogenic commitment and differentiation of human mesenchymal stem cells by low-intensity pulsed ultrasound stimulation. pubmed:28621452 |
Materials Science & Engineering C-Materials for Biological Applications | BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model Pubmed:30033316 |
Journal of Functional Foods | Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats 10.1016:j.jff.2018.04.048 |
Journal of Ethnopharmacology | Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model Pubmed:29705515 |
Life Sciences | Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway Pubmed:29883721 |
PLoS One | Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588 |
Royal Society of Chemistry | Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances c7tb02620j |
Molecular Medicine Reports | Effects of constrained dynamic loading, CKIP‑1 gene knockout and combination stimulations on bone loss caused by mechanical unloading Pubmed:29956799 |
Journal of Ultrasound in Medicine | A Comparison of 1‐and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study Pubmed:29781183 |
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American Scientific Publishers | Effects of KLD-12/Recombinant Human Bone Morphogenetic Protein-2 Nanofiber Scaffold on the Osteogenic Activity of Rabbit Bone Marrow Mesenchymal Stem Cells 10.1166:jbt.2018.1719 |
Journal of Materials Chemistry B | Enhanced osteoinductivity of porous biphasic calcium phosphate ceramic beads with high content of strontium incorporated calcium deficient hydroxyapatite Doi: 10.1039/C8TB01554F |
International Journal of Clinical and Experimental Medicine | Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats |
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Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats Pubmed: 30292771 |
Tropical Journal of Pharmaceutical Research | Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway |
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Advanced Healthcare Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809 |
American Journal of Translational Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 |
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Tropical Journal of Pharmaceutical Research | Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats. |
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Toxicology and applied pharmacology | Alpha ketoglutarate exerts a pro-osteogenic effect in osteoblast cell lines through activation of JNK and mTOR/S6K1/S6 signaling pathways Pubmed: 31051157 |
Journal of Innovations in Pharmaceutical and Biological Sciences | Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and … |
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Bulletin of Experimental Biology and Medicine | Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone Graft Pubmed: 31630302 |
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Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209 |
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J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway 33950533 |
Biochemical Engineering Journal | Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate |
Br J Pharmacol | Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis 34233019 |
PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism 34511043 |
Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway 34512780 |
ACS Appl Mater Interfaces | Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic Nanomedicine Pubmed:35311248 |
Molecules | Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells Pubmed:35684342 |
Journal of Exercise Science & Fitness | Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young Females Pubmed:35096081 |
Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing |
Catalog No. | Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species | Applications (RESEARCH USE ONLY!) |
RPA471Rb01 | Recombinant Osteocalcin (OC) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA471Rb51 | Polyclonal Antibody to Osteocalcin (OC) | WB; IHC; ICC; IP. |
PAA471Rb01 | Polyclonal Antibody to Osteocalcin (OC) | WB; IHC; ICC; IP. |
MAA471Rb21 | Monoclonal Antibody to Osteocalcin (OC) | WB; IHC; ICC; IP. |
SEA471Rb | ELISA Kit for Osteocalcin (OC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA471Rb | Multiplex Assay Kit for Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |