Multiplex Assay Kit for Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay)

ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 92-104 99
heparin plasma(n=5) 81-92 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 78-89% 96-105% 82-103%
EDTA plasma(n=5) 79-93% 79-95% 85-92% 86-97%
heparin plasma(n=5) 84-101% 98-105% 94-104% 92-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SHBG) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Nutrition and Cancer A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886
Journal of Life Science Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric Syndrome SMGHBM_2016_v26n9_1063
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
Toxicology and Applied Pharmacology Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male rats pubmed:27746313
中国医药导报 益肾祛浊方对PCOS-IR大鼠Ghrelin、SHBG及VF的影响 yycyzx201733003
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Biomed Pharmacother Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis 34029952
Food Science and Biotechnology Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats 33868755
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA396Hu61 Eukaryotic Sex Hormone Binding Globulin (SHBG) Positive Control; Immunogen; SDS-PAGE; WB.
EPA396Hu62 Eukaryotic Sex Hormone Binding Globulin (SHBG) Positive Control; Immunogen; SDS-PAGE; WB.
RPA396Hu02 Recombinant Sex Hormone Binding Globulin (SHBG) Positive Control; Immunogen; SDS-PAGE; WB.
RPA396Hu01 Recombinant Sex Hormone Binding Globulin (SHBG) Positive Control; Immunogen; SDS-PAGE; WB.
EPA396Hu63 Eukaryotic Sex Hormone Binding Globulin (SHBG) Positive Control; Immunogen; SDS-PAGE; WB.
APA396Hu61 Active Sex Hormone Binding Globulin (SHBG) Cell culture; Activity Assays.
APA396Hu62 Active Sex Hormone Binding Globulin (SHBG) Cell culture; Activity Assays.
PAA396Hu01 Polyclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
PAA396Hu02 Polyclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
LAA396Hu71 Biotin-Linked Polyclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC.
LAA396Hu81 FITC-Linked Polyclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IF.
MAA396Hu27 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
MAA396Hu29 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
MAA396Hu25 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB
MAA396Hu24 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
MAA396Hu26 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
MAA396Hu22 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
MAA396Hu21 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
MAA396Hu23 Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) WB; IHC; ICC; IP.
SEA396Hu ELISA Kit for Sex Hormone Binding Globulin (SHBG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA396Hu Multiplex Assay Kit for Sex Hormone Binding Globulin (SHBG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA396Hu01 ELISA Kit DIY Materials for Sex Hormone Binding Globulin (SHBG) Main materials for "Do It(ELISA Kit) Yourself"