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Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay)
AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA220Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 405
US$ 420
US$ 443
US$ 475
US$ 506
US$ 552
US$ 622
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 94-101 | 98 |
EDTA plasma(n=5) | 78-103 | 97 |
heparin plasma(n=5) | 78-90 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 97-105% | 84-97% | 95-103% | 91-99% |
EDTA plasma(n=5) | 88-97% | 95-102% | 84-102% | 80-99% |
heparin plasma(n=5) | 86-98% | 92-101% | 93-101% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:ADM) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Periodontal Research | Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2 Wiley: Source |
J Periodontal Res. | Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patients Pubmed: 23898869 |
Journal of Biosciences | Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen. Pubmed: 23660671 |
Journal of Translational Medicine | DNA methylation patterns in newborns exposed to tobacco in utero Pubmed:25623364 |
Pediatric Hematology and Oncology | Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and Procalcitonin PubMed: 26271020 |
Biogerontology. | Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia. pubmed:28501895 |
Journal of Clinical Investigation | Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366 |
Clinical Pathology and Medical Laboratory | ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS : |
Phytomedicine | Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failure Doi: 10.1016/j.phymed.2018.09.015 |
Biomedical Journal of Scientific & Technical Research | Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress? |
博士论文 | Clinical and experimental investigation of the nervous system of neonatal foals. Neuroendocrin transition from foetal consciousness to perinatal life |
Biology | Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy 34356497 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
APA220Mu01 | Active Adrenomedullin (ADM) | Cell culture; Activity Assays. |
RPA220Mu02 | Recombinant Adrenomedullin (ADM) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA220Mu01 | Recombinant Adrenomedullin (ADM) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA220Mu02 | Polyclonal Antibody to Adrenomedullin (ADM) | WB; IHC; ICC; IP. |
PAA220Mu01 | Polyclonal Antibody to Adrenomedullin (ADM) | WB; IHC; ICC; IP. |
CEA220Mu | ELISA Kit for Adrenomedullin (ADM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA220Mu | Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |