Packages (Simulation)
Reagent Preparation
Image (I)
Image (II)
Certificate
Multiplex Assay Kit for Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay)
IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA074Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range48.83-50000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 16.277 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 405
US$ 420
US$ 443
US$ 475
US$ 506
US$ 552
US$ 622
Result
For more details, please contact local distributors! US$ 778 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 87-99 | 94 |
EDTA plasma(n=5) | 79-89 | 83 |
heparin plasma(n=5) | 93-101 | 96 |
sodium citrate plasma(n=5) | 96-103 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-93% | 93-101% | 79-101% | 95-102% |
EDTA plasma(n=5) | 89-97% | 95-102% | 96-105% | 85-104% |
heparin plasma(n=5) | 78-97% | 87-95% | 79-95% | 85-103% |
sodium citrate plasma(n=5) | 95-105% | 96-104% | 96-105% | 78-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:IgG1) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
Magazine | Citations |
BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source |
Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861 |
Cellular Immunology | IgG autoantibody subclasses altered in immuno-related hemocytopenia Pubmed:25666506 |
Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394 |
Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847 |
Biomedicine & Pharmacotherapy | Icariin stimulates osteogenic differentiation of rat bone marrow stromal stem cells by increasing TAZ expression pubmed:28486190 |
Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397 |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605 |
Veterinary Microbiology | Optimized GAPDH-truncated immunogen of Streptococcus equi elicits an enhanced immune response and provides effective protection in a mouse model 33647714 |
British Journal of Pharmacology | The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
NPA074Mu01 | Native Immunoglobulin G1 (IgG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA074Mu01 | Recombinant Immunoglobulin G1 (IgG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA074Mu02 | Recombinant Immunoglobulin G1 (IgG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA074Mu21 | OVA Conjugated Immunoglobulin G1 (IgG1) | Immunogen; SDS-PAGE; WB. |
CPA074Mu22 | OVA Conjugated Immunoglobulin G1 (IgG1) | Immunogen; SDS-PAGE; WB. |
PAA074Mu02 | Polyclonal Antibody to Immunoglobulin G1 (IgG1) | WB; IHC; ICC; IP. |
PAA074Mu08 | Polyclonal Antibody to Immunoglobulin G1 (IgG1) | WB; IHC; ICC; IP. |
PAA074Mu01 | Polyclonal Antibody to Immunoglobulin G1 (IgG1) | WB; IHC; ICC; IP. |
PAA074Mu09 | Polyclonal Antibody to Immunoglobulin G1 (IgG1) | WB; IHC; ICC; IP. |
LAA074Mu81 | FITC-Linked Polyclonal Antibody to Immunoglobulin G1 (IgG1) | WB; IHC; ICC; IF. |
CEA074Mu | ELISA Kit for Immunoglobulin G1 (IgG1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA074Mu | Multiplex Assay Kit for Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |