Multiplex Assay Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay)

ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-101 92
EDTA plasma(n=5) 80-92 87
heparin plasma(n=5) 79-90 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 84-97% 90-101% 96-104%
EDTA plasma(n=5) 88-95% 97-105% 82-103% 90-97%
heparin plasma(n=5) 78-91% 78-95% 95-105% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:GDNF) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
 Molecular Neurobiology Efficient Generation of Functionally Active Spinal Cord Neurons from Spermatogonial Stem Cells pubmed:27566610
Chinese Medicial Journal Safflower Extract and Aceglutamide Injection Promoting Recovery of Peripheral Innervations via Vascular Endothelial Growth Factor-B Signaling in Diabetic Mice PMC5717862
The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg Acupuncture Alters Pro-infl ammatory Cytokines in the Plasma of Maternally Separated Rat Pups pubmed:28986807
Heroin Addiction And Related Clinical Problems GDNF serum levels are found to be lower in opioid-maintained patients ISSN 2531-4122
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Frontiers in Aging Neuroscience The Effects of Nordic Walking With Poles With an Integrated Resistance Shock Absorber on Cognitive Abilities and Cardiopulmonary Efficiency in … Pubmed: 33192480
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­ 33389158
Int J Biol Macromol The effect of biodegradable silk fibroin-based scaffolds containing glial cell line-derived neurotrophic factor (GDNF) on the corneal regeneration process 34119551
Inflammopharmacology Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway Pubmed:35366745
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
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APA043Mu01 Active Glial Cell Line Derived Neurotrophic Factor (GDNF) Cell culture; Activity Assays.
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RPA043Mu01 Recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA043Mu01 Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF) WB; IHC; ICC; IP.
PAA043Mu02 Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF) WB; IHC; ICC; IP.
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SEA043Mu ELISA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA043Mu Mini Samples ELISA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA043Mu CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) Chemiluminescent immunoassay for Antigen Detection.
LMA043Mu Multiplex Assay Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.