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DAB staining on IHC-P;
Samples: Porcine Cardiac Muscle Tissue
Primary Ab: 20µg/ml Biotin-Linked Polyclonal Antibody to Apelin 12 (AP12)
Second Ab: 2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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Biotin-Linked Polyclonal Antibody to Apelin 12 (AP12)
APLN12
- Product No.LAA856Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 104
US$ 242
US$ 346
US$ 865
US$ 3460
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against AP12. It has been selected for its ability to recognize AP12 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Clinical and Experimental Hypertension | Serum concentrations of apelin-17 isoform vary in accordance to blood pressure categories in individuals with obesity class 3 Pubmed:29652188 |
| Pediatr Diabetes | Adropin and apelin‐12 efficiently predict metabolic syndrome in obese children Pubmed: 32749012 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| PAA856Hu01 | Polyclonal Antibody to Apelin 12 (AP12) | WB; IHC; ICC; IP. |
| LAA856Hu71 | Biotin-Linked Polyclonal Antibody to Apelin 12 (AP12) | WB; IHC; ICC. |
| CEA856Hu | ELISA Kit for Apelin 12 (AP12) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA856Hu | CLIA Kit for Apelin 12 (AP12) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA856Hu | Multiplex Assay Kit for Apelin 12 (AP12) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA856Hu11 | ELISA Kit DIY Materials for Apelin 12 (AP12) | Main materials for "Do It (ELISA Kit) Yourself". |