Biotin-Linked Polyclonal Antibody to Collagen Type II (COL2)
- Product No.LAA572Gu71
- Organism SpeciesCavia (Guinea pig ) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 132
For more details, please contact local distributors! US$ 308 US$ 440 US$ 1100 US$ 4400
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against COL2. It has been selected for its ability to recognize COL2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine | Citations |
Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069 |
Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658 |
Spine (Phila Pa 1976). | No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes. Pubmed:25856264 |
PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592 |
Materials Science and Engineering: C | Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition Pubmed:24433895 |
義守大學生物技術與化學工程研究所學位論文 | 可拋棄式退化性關節炎電化學式尿液免疫感測器之研究 Electrochemical Immunosensor Detection of Urinary Biomaker for Osteoarthritis Diagnosis Publication: U0074-2807201500521300 |
Biomed Rep | Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs PubMed: 26623021 |
Macromolecular Bioscience | Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871 |
Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310 |
Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063 |
中国眼耳鼻喉科杂志 | 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响 article:11593 |
Biomedical Materials | An innovative co-axial system to electrospin in situ crosslinked gelatin nanofibers. Pubmed:26987305 |
Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds pubmed:27821864 |
PLoS One. | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788 |
Polymers | Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering 2073-4360:8 |
PLOS ONE | Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders 10.1371:journal.pone.0187564 |
PLoS One | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders 10.1371:journal.pone.0172181 |
Platelets. | Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate. pubmed:29090639 |
Molecular Medicine Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits Pubmed:29257344 |
Journal of Materials Science | Scaffolds modified with graphene as future implants for nasal cartilage |
Processes | A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation |
Catalog No. | Related products for research use of Cavia (Guinea pig ) Organism species | Applications (RESEARCH USE ONLY!) |
PAA572Gu01 | Polyclonal Antibody to Collagen Type II (COL2) | WB; IHC; ICC; IP. |
LAA572Gu71 | Biotin-Linked Polyclonal Antibody to Collagen Type II (COL2) | WB; IHC; ICC. |