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High Sensitive ELISA Kit for Apolipoprotein B48 (APOB48)
Apo-B48
- Product No.HEB883Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range2.4-150ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.1ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 512
US$ 732
US$ 3294
US$ 6222
US$ 51240
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of High Sensitive Apolipoprotein B48 (APOB48).
No significant cross-reactivity or interference between High Sensitive Apolipoprotein B48 (APOB48) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Apolipoprotein B48 (APOB48) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Apolipoprotein B48 (APOB48) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-91 | 88 |
| EDTA plasma(n=5) | 86-101 | 95 |
| heparin plasma(n=5) | 98-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Apolipoprotein B48 (APOB48) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Apolipoprotein B48 (APOB48) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Apolipoprotein B48 (APOB48) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 83-90% | 87-94% | 78-89% |
| EDTA plasma(n=5) | 79-94% | 82-101% | 94-104% | 85-104% |
| heparin plasma(n=5) | 79-93% | 92-99% | 98-105% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Steroids | Effects of Anabolic Androgenic Steroids on Chylomicron Metabolism PubMed: 22939845 |
| Human Health and Nutritional Sciences | The Influences of Pectin and Apples, as a Pectin-rich Food Matrix, on Lipid Digestibility, Bioaccessibility and Postprandial Lipemia |
| British Journal of Nutrition | Acute whole apple consumption did not influence postprandial lipemia: A randomized crossover trial Pubmed: 31902373 |
| Clinical Nutrition | Postprandial dyslipidemia after a standardized high-fat meal in BMI-matched healthy individuals, and in subjects with prediabetes or Type 2 diabetes 34656950 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB883Hu01 | Recombinant Apolipoprotein B48 (APOB48) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB883Hu02 | Recombinant Apolipoprotein B48 (APOB48) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB883Hu01 | Polyclonal Antibody to Apolipoprotein B48 (APOB48) | WB; IHC; ICC; IP. |
| MAB883Hu21 | Monoclonal Antibody to Apolipoprotein B48 (APOB48) | WB; IHC; ICC; IP. |
| SEB883Hu | ELISA Kit for Apolipoprotein B48 (APOB48) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB883Hu | High Sensitive ELISA Kit for Apolipoprotein B48 (APOB48) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB883Hu | CLIA Kit for Apolipoprotein B48 (APOB48) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB883Hu | Multiplex Assay Kit for Apolipoprotein B48 (APOB48) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |