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High Sensitive ELISA Kit for Oxytocin (OT)

Pitocin; Syntocinon

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Oxytocin (OT).
No significant cross-reactivity or interference between High Sensitive Oxytocin (OT) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of High Sensitive Oxytocin (OT) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Oxytocin (OT) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-98 87
EDTA plasma(n=5) 92-101 96
heparin plasma(n=5) 91-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Oxytocin (OT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Oxytocin (OT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Oxytocin (OT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-103% 86-103% 82-95% 93-102%
EDTA plasma(n=5) 85-97% 87-94% 88-105% 95-102%
heparin plasma(n=5) 84-98% 96-105% 80-92% 84-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
J Psychiatr Res.? Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individuals Pubmed:24411593
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study Pubmed:27143518
Progress in neuro-psychopharmacology & biological psychiatry Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect. pubmed:28093220
International Journal of Clinical and Experimental Medicine Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … files:ijcem0031358.pdf
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study pubmed:27143518
BMC Med. Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial. pubmed:28209155
Psychiatry Research Plasma oxytocin levels in major depressive and bipolar II disorders pubmed:28865715
BMC Medicine Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial 10.1186:s12916-017-0801-0
EUROPEAN ADDICTION RESEARCH The Association between the Level of Plasma Oxytocin and Craving among Former Heroin Users Pubmed:29902803
ASN Neuro . Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup Deprivation Pubmed: 32962418
European Journal of Integrative Medicine Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …
J. Pers. Med Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Naïve Major Depressive Patients
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