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Multiplex Assay Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array)
LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPARGC1; Ligand effect modulator 6
- Product No.CMH337Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.0065ng/mL
- Sensitivityn/a
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$
US$
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-89 | 86 |
| EDTA plasma(n=5) | 81-103 | 91 |
| heparin plasma(n=5) | 98-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-101% | 96-104% | 80-99% | 98-105% |
| EDTA plasma(n=5) | 85-95% | 79-95% | 87-94% | 99-105% |
| heparin plasma(n=5) | 88-96% | 90-103% | 97-105% | 78-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| Coronary Artery Disease | Impaired myocardium energetics associated with the risk for new-onset atrial fibrillation after isolated coronary artery bypass graft surgery Pubmed: 24463787 |
| Molecular and cellular biochemistry | Skeletal muscle mitochondrial dysfunction precedes right ventricular impairment in experimental pulmonary hypertension Pubmed: 23099843 |
| Diabetes. | Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574 |
| Psychoneuroendocrinology | Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats PubMed: 26143539 |
| Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | The level of circulating PGC1α in cardiovascular diseases Article: 10.1134 |
| Neuroscience Letters | Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosis pubmed:28483651 |
| The American Journal of the Medical Sciences | Serum peroxisome proliferator-activated receptor gamma coactivator-1α related to myocardial energy expenditure in patients with chronic heart failure Doi: 10.1016/j.amjms.2018.12.002 |
| Brain Research | The effect of antecedent-conditioning high-intensity interval training on BDNF regulation through PGC-1α pathway following cerebral ischemia Pubmed: 31866362 |
| Food & Function | Ubiquinol supplementation modulates energy metabolism and bone turnover during high intensity exercise Pubmed: 32797125 |
| Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis | |
| CLINICAL NUTRITION | Long lasting protective EFFECTS of early L-ARGININE TREAMENT on ENDOTHELIUM in an in Vitro STUDY 33743287 |
| Nature Communications | Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024 |