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Multiplex Assay Kit for Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array)
C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16
- Product No.CMC064Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids.
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.9766-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.3255pg/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 638
US$ 912
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-90 | 86 |
| EDTA plasma(n=5) | 97-105 | 101 |
| heparin plasma(n=5) | 95-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 24 (IL24) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-94% | 79-97% | 78-102% | 83-93% |
| EDTA plasma(n=5) | 82-95% | 78-97% | 97-105% | 80-97% |
| heparin plasma(n=5) | 82-96% | 79-97% | 83-102% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| Experimental & Clinical Cancer Research | Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo PubMed: PMC3511263 |
| Bioresources and Bioprocessing | Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies Springer:Source |
| Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688 |
| Theranostics | IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer 33456560 |
| BIOLOGICAL CHEMISTRY | IL-24 inhibits the malignancy of human glioblastoma cells via destabilization of Zeb1 33894112 |
| J Transl Med | Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases 34078403 |
| Nutrients | Effect of Serum Spermidine on the Prognosis in Patients with Acute Myocardial Infarction: A Cohort Study Pubmed:35406007 |