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Multiplex Assay Kit for Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array)

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-92 84
EDTA plasma(n=5) 78-101 82
heparin plasma(n=5) 87-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 93-104% 94-105% 79-101%
EDTA plasma(n=5) 85-93% 81-104% 97-104% 80-101%
heparin plasma(n=5) 82-103% 91-99% 78-90% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
allergy and clinical immunology Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis Siaic-Review:Source
BMC Immunol Increased levels of interleukin 31 (IL-31) in osteoporosis PubMed: 2644965
Sci Rep. Interleukin-31 expression and relation to disease severity in human asthma pmc:PMC4783779
Exp Dermatol. Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice. pubmed:28191683
Nat Commun.  The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. pubmed:28067314
Experimental Dermatology Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice doi:10.1111
アトピー性皮膚炎の痒みの発症機序に関する研究
Indian Journal of Public Health Research & Development Determination the Role of Interleukin 31 (IL-31) Levels in Three Real Allergic Diseases (Asthma, Rhinitis, Urticaria)
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
EPB179Ca63 Eukaryotic Interleukin 31 (IL31) Positive Control; Immunogen; SDS-PAGE; WB.
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CMB179Ca Multiplex Assay Kit for Interleukin 31 (IL31) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
LMB179Ca Multiplex Assay Kit for Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.