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Multiplex Assay Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array)

CD354; Triggering Receptor Expressed On Monocytes-1

Specificity

This assay has high sensitivity and excellent specificity for detection of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-98 91
EDTA plasma(n=5) 93-105 101
heparin plasma(n=5) 90-101 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 82-97% 78-103% 92-99%
EDTA plasma(n=5) 80-101% 78-96% 97-104% 79-96%
heparin plasma(n=5) 86-101% 97-104% 78-95% 82-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
Journal of Pharmacology and Experimental Therapeutics Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar Macrophages Aspet: 171462
Arthritis Research & Therapy Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine. pubmed:27624724
Chinese medical journal Role of Triggering Receptor Expressed on Myeloid Cell-1 Expression in Mammalian Target of Rapamycin Modulation of CD8+ T-cell Differentiation during the Immune Response to Invasive Pulmonary Aspergillosis PMC5443028
Journal of Maternal-Fetal & Neonatal Medicine Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state Pubmed:29534643
Journal of Maternal-Fetal & Neonatal Medicine A promising novel biomarker for early-onset preeclampsia: soluble trigger receptor expressed on myeloid cells-1 (sTREM-1) Pubmed: 33207989
Ioannis Gkougkourellas, Alexandros Sarantopoulos, Konstantinos Tselios, Athanasios Kalogeridis
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