≡

Multiplex Assay Kit for Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array)

CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11

Specificity

This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-103 89
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 91-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 78-102% 91-101% 91-98%
EDTA plasma(n=5) 92-101% 78-98% 91-99% 98-105%
heparin plasma(n=5) 84-101% 97-104% 99-105% 87-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY Elevated Serum Level of CD48 in Patients with Intermittent Allergic Rhinitis Pubmed: 32966985
Exp Mol Pathol The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­ 33285209
Immunopharmacol Immunotoxicol sCD48 is elevated in non-allergic but not in allergic persistent rhinitis 34477021
Exp Eye Res Sulforaphane inhibits cytokine-stimulated chemokine and adhesion molecule expressions in human corneal fibroblasts: Involvement of the MAPK, STAT, and NF-κB … Pubmed:35038457
Catalog No. Related products for research use of Cavia (Guinea pig ) Organism species Applications (RESEARCH USE ONLY!)
RPA025Gu01 Recombinant Eosinophil Chemotactic Factor (ECF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA025Gu01 Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF) Testing in progress
MAA025Gu21 Monoclonal Antibody to Eosinophil Chemotactic Factor (ECF) WB; IHC; ICC; IP.
SEA025Gu ELISA Kit for Eosinophil Chemotactic Factor (ECF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA025Gu CLIA Kit for Eosinophil Chemotactic Factor (ECF) Chemiluminescent immunoassay for Antigen Detection.
LMA025Gu Multiplex Assay Kit for Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMA025Gu Multiplex Assay Kit for Eosinophil Chemotactic Factor (ECF) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.