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ELISA Kit for Salusin Alpha (SALa)

Specificity

This assay has high sensitivity and excellent specificity for detection of Salusin Alpha (SALa).
No significant cross-reactivity or interference between Salusin Alpha (SALa) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Salusin Alpha (SALa) and the recovery rates were calculated by comparing the measured value to the expected amount of Salusin Alpha (SALa) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-95 91
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 80-91 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salusin Alpha (SALa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salusin Alpha (SALa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Salusin Alpha (SALa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 96-103% 88-102% 96-104%
EDTA plasma(n=5) 88-104% 86-94% 94-103% 79-91%
heparin plasma(n=5) 98-105% 80-94% 78-101% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Peptides The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass PubMed: 22884920
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533
European Journal of Obstetrics & Gynecology and Reproductive Biology Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. PubMed: 23178004
Biotechnic & Histochemistry Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Pubmed: 23957705
Peptides Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus ScienceDirect: S0196978113003100
Med Sci Monit. Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome Pubmed:Pmc4136931
Pediatr Nephrol. Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? Pubmed:25245503
European Journal of Dermatology Serum salusin-α and salusin-β levels in patients with psoriasis PubMed: 26055619
Gynecol Endocrinol Serum salusins levels are increased and correlated positively with cyst size in ovarian endometrioma PubMed: 26008602
Multiple Sclerosis and Related Disorders Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis Pubmed: 30738876
cardiology in the young Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity Pubmed: 31486354
Drug and Chemical Toxicology Salusin-α mitigates diabetic nephropathy via inhibition of the Akt/mTORC1/p70S6K signaling pathway in diabetic rats Pubmed: 31665937
Journal of international medical research Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis Pubmed: 32054363
J Ultrasound Med Targeted Delivery of Salusin©\¦Á into Rabbit Carotid Arterial Endothelium Using SonoVue 33818784
Food Science & Nutrition Inhibition of oxidative stress and apoptosis by camel milk mitigates cyclosporine©\induced nephrotoxicity: Targeting Nrf2/HO©\1 and AKT/eNOS/NO pathways 34136182
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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