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ELISA Kit for Neuromedin B (NMB)

NM-B

Specificity

This assay has high sensitivity and excellent specificity for detection of Neuromedin B (NMB).
No significant cross-reactivity or interference between Neuromedin B (NMB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neuromedin B (NMB) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuromedin B (NMB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-102 98
EDTA plasma(n=5) 78-92 82
heparin plasma(n=5) 79-98 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuromedin B (NMB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuromedin B (NMB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuromedin B (NMB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-91% 78-94% 93-101% 94-104%
EDTA plasma(n=5) 82-93% 79-105% 80-89% 86-93%
heparin plasma(n=5) 86-102% 78-92% 79-93% 80-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Endocrinology Expression and regulation of neuromedin B in pituitary corticotrophs of male melanocortin 2 receptor-deficient mice Pubmed: 24742195
PLoS One Neuromedin B Restores Erectile Function by Protecting the Cavernous Body and the Nitrergic Nerves from Injury in a Diabetic Rat Model PubMed: 26207818
american journal of hematology Impact of natural Neuromedin‐B receptor variants on iron metabolism Pubmed: 31724192
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA803Ra01 Recombinant Neuromedin B (NMB) Positive Control; Immunogen; SDS-PAGE; WB.
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MAA803Ra21 Monoclonal Antibody to Neuromedin B (NMB) WB; IHC; ICC; IP.
CEA803Ra ELISA Kit for Neuromedin B (NMB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA803Ra Multiplex Assay Kit for Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.