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ELISA Kit for Folic Acid (FA)
VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate
- Product No.CEA610Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range781.25-200,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 285.77pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 557
US$ 796
US$ 3580
US$ 6763
US$ 55692
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Folic Acid (FA).
No significant cross-reactivity or interference between Folic Acid (FA) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Folic Acid (FA) and the recovery rates were calculated by comparing the measured value to the expected amount of Folic Acid (FA) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 98 |
| EDTA plasma(n=5) | 79-90 | 86 |
| heparin plasma(n=5) | 80-89 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folic Acid (FA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folic Acid (FA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Folic Acid (FA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-93% | 83-92% | 98-105% | 99-105% |
| EDTA plasma(n=5) | 94-105% | 97-105% | 91-98% | 78-90% |
| heparin plasma(n=5) | 79-95% | 79-94% | 90-99% | 93-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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| Magazine | Citations |
| Annals of Surgical Oncology | Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients. pubmed:29058145 |
| ACS Applied Materials & Interfaces | Folic Acid-Functionalized Hybrid Photonic Barcodes for Capture and Release of Circulating Tumor Cells Pubmed:29882648 |
| Journal of Pharmaceutical Sciences | Development of Orally Applicable, Combinatorial Drug–Loaded Nanoparticles for the Treatment of Fibrosarcoma Pubmed:29339136 |
| Aging Male | Role of folic acid deficiency as a possible risk factor for erectile dysfunction Pubmed: 29157083 |
| International Urology and Nephrology | Low serum folic acid can be a potential independent risk factor for erectile dysfunction: a prospective case–control study Pubmed: 30547361 |
| Int J Biol Macromol | Enhanced cellular uptake, transport and oral bioavailability of optimized folic acid-loaded chitosan nanoparticles Pubmed:35292282 |
| Frontiers in Nutrition | Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, Ethiopia Pubmed:35464038 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA610Ge11 | BSA Conjugated Folic Acid (FA) | Immunogen; SDS-PAGE; WB. |
| CPA610Ge21 | OVA Conjugated Folic Acid (FA) | Immunogen; SDS-PAGE; WB. |
| CPA610Ge41 | HSA Conjugated Folic Acid (FA) | Immunogen; SDS-PAGE; WB. |
| PAA610Ge01 | Polyclonal Antibody to Folic Acid (FA) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA610Ge81 | FITC-Linked Polyclonal Antibody to Folic Acid (FA) | WB; IHC; ICC; IF. |
| LAA610Ge71 | Biotin-Linked Polyclonal Antibody to Folic Acid (FA) | WB; IHC; ICC. |
| CEA610Ge | ELISA Kit for Folic Acid (FA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA610Ge | CLIA Kit for Folic Acid (FA) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA610Ge | Multiplex Assay Kit for Folic Acid (FA) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA610Ge11 | ELISA Kit DIY Materials for Folic Acid (FA) | Main materials for "Do It (ELISA Kit) Yourself". |