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ELISA Kit for Growth Hormone Releasing Hormone (GHRH)
GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin
- Product No.CEA438Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.59pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 454
US$ 648
US$ 2916
US$ 5508
US$ 45360
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH).
No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Growth Hormone Releasing Hormone (GHRH) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone Releasing Hormone (GHRH) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 92-99 | 96 |
| heparin plasma(n=5) | 81-101 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone Releasing Hormone (GHRH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-102% | 88-96% | 87-95% | 80-101% |
| EDTA plasma(n=5) | 94-103% | 81-99% | 94-101% | 99-105% |
| heparin plasma(n=5) | 96-105% | 89-99% | 84-99% | 88-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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| Magazine | Citations |
| 19 | Role of GHRH in Sleep and Growth Impairments Induced by Upper Airway Obstruction in Rats Ers: 00197610 |
| 19 | Role of growth hormone-releasing hormone in sleep and growth impairments induced by upper airway obstruction in rats PubMed: 21406516 |
| Global Veterinaria | Effect of Moringa Oleifera Extract on Nicotine Induced Neurotoxicity in Female Rat and Their Embryos Idosi: Source |
| Cell Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008 |
| Drug Testing and Analysis | An Immuno‐PCR screen for the detection of CJC‐1295 and other Growth Hormone Releasing Hormone analogues in equine plasma Pubmed: 30489688 |
| Sleep and Breathing | Effects of somatotropic axis on cognitive dysfunction of obstructive sleep apnea Pubmed: 31073904 |
| Diabetes | Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus. 33980693 |
| Sci Rep | Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients Pubmed:35508633 |