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ELISA Kit for Activin B (ACVB)

ACV-B; Activin Beta B Beta B Homodimer

Specificity

This assay has high sensitivity and excellent specificity for detection of Activin B (ACVB).
No significant cross-reactivity or interference between Activin B (ACVB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Activin B (ACVB) and the recovery rates were calculated by comparing the measured value to the expected amount of Activin B (ACVB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-102 99
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 80-99 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin B (ACVB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin B (ACVB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin B (ACVB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-90% 85-104% 98-105% 91-99%
EDTA plasma(n=5) 90-97% 78-93% 93-104% 87-101%
heparin plasma(n=5) 90-104% 90-98% 78-101% 89-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Gastroenterology Research and Practice Serum Activins and Follistatin during the Treatment of Chronic Hepatitis C Genotypes 1 and 4 and Their Correlations with Viral Load and Liver Enzymes: A Preliminary Report. Pubmed: 24799891
Gastroenterol Res Pract.? Serum activins and follistatin during the treatment of chronic hepatitis c genotypes 1 and 4 and their correlations with viral load and liver enzymes: A preliminary report Pubmed:Pmc3995172
Clin Exp Med.? Effects?of?chronic hepatitis C?genotype?1?and 4 on serum activins and follistatin in treatment na?ve patients and their correlations with interleukin-6, tumour necrosis factor-α, viral load and liver damage. Pubmed:24925642
Clin Biochem Serum activin B concentration as predictive biomarker for ectopic pregnancy Pubmed:26968108
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986
Australasian Journal of Ultrasound in Medicine The potential value of activin B and fibronectin for the triage of pregnancies of unknown location and prediction of first trimester viability 10.1002:ajum.12090
Pathology & Oncology Research Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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