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Western Blot; Sample: A549 cell lysate
Primary Ab: 0.2µg/ml Mouse Anti-Human H3 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Anti-Histone H3 (H3) Monoclonal Antibody
- Product No.CAA285Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreOryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog)
- ApplicationsLoading Control of WB.
- Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 119
US$ 279
US$ 398
US$ 995
US$ 3980
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Biomed Res Int | A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890 |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA285Hu01 | Active Histone H3 (H3) | Cell culture; Activity Assays. |
| RPA285Hu01 | Recombinant Histone H3 (H3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA285Hu01 | Anti-Histone H3 (H3) Polyclonal Antibody | Loading Control of WB. |
| PAA285Hu01 | Polyclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| CAA285Hu22 | Anti-Histone H3 (H3) Monoclonal Antibody | Loading Control of WB. |
| KSA285Hu01 | ELISA Kit DIY Materials for Histone H3 (H3) | Main materials for "Do It (ELISA Kit) Yourself". |