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ELISA Kit for Anti-Nephrin Antibody (Anti-NPHN)
NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor
- Product No.AEA937Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodIndirect ELISA
- Assay Length2h, 30min
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.22ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 630
For more details, please contact local distributors! US$ 900 US$ 4050 US$ 7650 US$ 63000
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-Nephrin Antibody (Anti-NPHN).
No significant cross-reactivity or interference between Anti-Nephrin Antibody (Anti-NPHN) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Anti-Nephrin Antibody (Anti-NPHN) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Nephrin Antibody (Anti-NPHN) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 95-103 | 99 |
EDTA plasma(n=5) | 91-101 | 95 |
heparin plasma(n=5) | 95-104 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Nephrin Antibody (Anti-NPHN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Nephrin Antibody (Anti-NPHN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Nephrin Antibody (Anti-NPHN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-99% | 86-94% | 82-92% | 80-102% |
EDTA plasma(n=5) | 89-99% | 95-105% | 85-94% | 78-96% |
heparin plasma(n=5) | 93-101% | 97-105% | 78-90% | 91-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Yonsei medical journal | The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. pubmed:28120572 |
Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788 |
British Journal of Haematology | Renal protection by atorvastatin in a murine model of sickle cell nephropathy Pubmed:29527679 |
Journal of Clinical Medicine | Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903 |
Disease Markers | Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724 |
Biomed Rep | Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs Pubmed:35620307 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA937Hu01 | Recombinant Nephrin (NPHN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA937Hu03 | Recombinant Nephrin (NPHN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA937Hu04 | Recombinant Nephrin (NPHN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA937Hu02 | Recombinant Nephrin (NPHN) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA937Hu01 | Polyclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
PAA937Hu03 | Polyclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
PAA937Hu02 | Polyclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
MAA937Hu22 | Monoclonal Antibody to Nephrin (NPHN) | WB; ICC/IF |
MAA937Hu21 | Monoclonal Antibody to Nephrin (NPHN) | WB |
MAA937Hu23 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
MAA937Hu24 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
MAA937Hu25 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
MAA937Hu27 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
MAA937Hu29 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
SEA937Hu | ELISA Kit for Nephrin (NPHN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA937Hu | ELISA Kit for Anti-Nephrin Antibody (Anti-NPHN) | Enzyme-linked immunosorbent assay for Antibody Detection. |
LMA937Hu | Multiplex Assay Kit for Nephrin (NPHN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |