Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
[Edit]TC-25; p21-Rac1; MIG5; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1
![Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)](/upload/item/img/Ras-Related-C3-Botulinum-Toxin-Substrate-1-(Rac1)-81427.jpg)
Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. This mutant did bind to POR1 and it induced membrane ruffling and transformation. A second RAC mutant, which bound PAK but not POR1, induced JNK activation but was defective in inducing membrane ruffling and transformation. The authors concluded that the effects of RAC on the JNK cascade and on actin polymerization and cell proliferation are mediated by distinct effector functions that diverge at the level of RAC itself.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPM427Hu01 | Recombinant Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAM427Hu21 | Monoclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | WB; IHC; ICC; IP. |
PAM427Hu01 | Polyclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | WB; IHC; ICC; IP. | |
Assay Kits | SEM427Hu | ELISA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCM427Hu | CLIA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Chemiluminescent immunoassay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPM427Mu01 | Recombinant Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | n/a | Monoclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | SEM427Mu | ELISA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPM427Ra01 | Recombinant Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAM427Ra01 | Polyclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | WB; IHC; ICC; IP. |
MAM427Ra21 | Monoclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | WB; IHC; ICC; IP. | |
Assay Kits | SEM427Ra | ELISA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
- "Rac, a novel ras-related family of proteins that are botulinum toxin substrates."J. Biol. Chem. 264:16378-16382(1989) [PubMed] [Europe PMC] [Abstract]
- "Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line."Mol. Cell. Biol. 10:1793-1798(1990) [PubMed] [Europe PMC] [Abstract]
- "Small GTPase Rac1: structure, localization, and expression of the human gene."Biochem. Biophys. Res. Commun. 277:741-751(2000) [PubMed] [Europe PMC] [Abstract]
- "Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors."Oncogene 18:6835-6839(1999) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA."J. Biol. Chem. 266:9786-9794(1991) [PubMed] [Europe PMC] [Abstract]
- "The small GTP-binding protein rac regulates growth factor-induced membrane ruffling."Cell 70:401-410(1992) [PubMed] [Europe PMC] [Abstract]
- "Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity."J. Biol. Chem. 270:22473-22477(1995) [PubMed] [Europe PMC] [Abstract]
- "The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization."Mol. Cell. Biol. 17:2247-2256(1997) [PubMed] [Europe PMC] [Abstract]
- "Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases."J. Biol. Chem. 273:34954-34960(1998) [PubMed] [Europe PMC] [Abstract]
- "Non-adherent cell-specific expression of DOCK2, a member of the human CDM-family proteins."Biochim. Biophys. Acta 1452:179-187(1999) [PubMed] [Europe PMC] [Abstract]
- "The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1."J. Cell Sci. 113:3267-3275(2000) [PubMed] [Europe PMC] [Abstract]
- "IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling."Nature 408:732-735(2000) [PubMed] [Europe PMC] [Abstract]
- "The semaphorin receptor plexin-B1 specifically interacts with active Rac in a ligand-dependent manner."Proc. Natl. Acad. Sci. U.S.A. 97:12457-12462(2000) [PubMed] [Europe PMC] [Abstract]
- "Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."Genes Cells 6:107-119(2001) [PubMed] [Europe PMC] [Abstract]
- "P-Rex1, a PtdIns(3,4,5)P3- and Gbetagamma-regulated guanine-nucleotide exchange factor for Rac."Cell 108:809-821(2002) [PubMed] [Europe PMC] [Abstract]
- "The integrin cytoplasmic domain-associated protein ICAP-1 binds and regulates Rho family GTPases during cell spreading."J. Cell Biol. 156:377-387(2002) [PubMed] [Europe PMC] [Abstract]
- "Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex."Nat. Cell Biol. 4:574-582(2002) [PubMed] [Europe PMC] [Abstract]
- "Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases."J. Biol. Chem. 278:25234-25246(2003) [PubMed] [Europe PMC] [Abstract]
- "The TRE17 oncogene encodes a component of a novel effector pathway for Rho GTPases Cdc42 and Rac1 and stimulates actin remodeling."Mol. Cell. Biol. 23:2151-2161(2003) [PubMed] [Europe PMC] [Abstract]
- "The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak."J. Biol. Chem. 279:32824-32831(2004) [PubMed] [Europe PMC] [Abstract]
- "The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2."FASEB J. 19:467-469(2005) [PubMed] [Europe PMC] [Abstract]
- "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
- "The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18."Neuron 51:727-739(2006) [PubMed] [Europe PMC] [Abstract]
- "Cooperation of DEF6 with activated Rac in regulating cell morphology."J. Biol. Chem. 282:2011-2018(2007) [PubMed] [Europe PMC] [Abstract]
- "Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties."J. Cell Sci. 120:1663-1672(2007) [PubMed] [Europe PMC] [Abstract]
- "Asef2 functions as a Cdc42 exchange factor and is stimulated by the release of an autoinhibitory module from a concealed C-terminal activation element."Mol. Cell. Biol. 27:1380-1393(2007) [PubMed] [Europe PMC] [Abstract]
- "Activated Rac1, but not the tumorigenic variant Rac1b, is ubiquitinated on Lys 147 through a JNK-regulated process."FEBS J. 275:386-396(2008) [PubMed] [Europe PMC] [Abstract]
- "Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease."Nat. Med. 14:1370-1376(2008) [PubMed] [Europe PMC] [Abstract]
- "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
- "The Rho-family GEF Asef2 activates Rac to modulate adhesion and actin dynamics and thereby regulate cell migration."J. Cell Sci. 122:4535-4546(2009) [PubMed] [Europe PMC] [Abstract]
- "The SWI/SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING finger protein Unkempt."FEBS J. 277:1453-1464(2010) [PubMed] [Europe PMC] [Abstract]
- "BPAG1e maintains keratinocyte polarity through beta4 integrin-mediated modulation of Rac1 and cofilin activities."Mol. Biol. Cell 20:2954-2962(2009) [PubMed] [Europe PMC] [Abstract]
- "The fic domain: regulation of cell signaling by adenylylation."Mol. Cell 34:93-103(2009) [PubMed] [Europe PMC] [Abstract]
- "AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling."Science 323:269-272(2009) [PubMed] [Europe PMC] [Abstract]
- "Armus is a Rac1 effector that inactivates Rab7 and regulates E-cadherin degradation."Curr. Biol. 20:198-208(2010) [PubMed] [Europe PMC] [Abstract]
- "Semaphorin 5A and plexin-B3 inhibit human glioma cell motility through RhoGDIalpha-mediated inactivation of Rac1 GTPase."J. Biol. Chem. 285:32436-32445(2010) [PubMed] [Europe PMC] [Abstract]
- "Activated Rac1 GTPase translocates protein phosphatase 5 to the cell membrane and stimulates phosphatase activity in vitro."J. Biol. Chem. 285:3872-3882(2010) [PubMed] [Europe PMC] [Abstract]
- "Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism."J. Cell Biol. 190:461-477(2010) [PubMed] [Europe PMC] [Abstract]
- "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
- "ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion."Biochem. Biophys. Res. Commun. 409:96-102(2011) [PubMed] [Europe PMC] [Abstract]
- "The E3 ubiquitin-ligase HACE1 catalyzes the ubiquitylation of active Rac1."Dev. Cell 21:959-965(2011) [PubMed] [Europe PMC] [Abstract]
- "The F-BAR domain protein PACSIN2 associates with Rac1 and regulates cell spreading and migration."J. Cell Sci. 124:2375-2388(2011) [PubMed] [Europe PMC] [Abstract]
- "The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells."Mol. Cell. Biol. 31:81-91(2011) [PubMed] [Europe PMC] [Abstract]
- "The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization."J. Cell Biol. 199:985-1001(2012) [PubMed] [Europe PMC] [Abstract]
- "ARHGDIA: a novel gene implicated in nephrotic syndrome."J. Med. Genet. 50:330-338(2013) [PubMed] [Europe PMC] [Abstract]
- "Beta-arrestin-dependent activation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6."Sci. Signal. 6:RA30-RA30(2013) [PubMed] [Europe PMC] [Abstract]
- "Structural basis for autoinhibition of the guanine nucleotide exchange factor FARP2."Structure 21:355-364(2013) [PubMed] [Europe PMC] [Abstract]
- "The crystal structure of human rac1, a member of the rho-family complexed with a GTP analogue."Nat. Struct. Biol. 4:147-152(1997) [PubMed] [Europe PMC] [Abstract]
- "Structure of the TPR domain of p67phox in complex with Rac.GTP."Mol. Cell 6:899-907(2000) [PubMed] [Europe PMC] [Abstract]
- "Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1."Nature 408:682-688(2000) [PubMed] [Europe PMC] [Abstract]
- "Modulation of host signaling by a bacterial mimic: structure of the Salmonella effector SptP bound to Rac1."Mol. Cell 6:1449-1460(2000) [PubMed] [Europe PMC] [Abstract]
- "How the Pseudomonas aeruginosa ExoS toxin downregulates Rac."Nat. Struct. Biol. 8:23-26(2001) [PubMed] [Europe PMC] [Abstract]
- "Crystal structure of the Rac1-RhoGDI complex involved in NADPH oxidase activation."Biochemistry 40:10007-10013(2001) [PubMed] [Europe PMC] [Abstract]
- "The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways."Nature 411:215-219(2001) [PubMed] [Europe PMC] [Abstract]
- "Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase."J. Biol. Chem. 279:4743-4749(2004) [PubMed] [Europe PMC] [Abstract]
- "Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors."Cell 126:869-880(2006) [PubMed] [Europe PMC] [Abstract]
- "Crystal structure of Rac1 bound to its effector phospholipase C-beta2."Nat. Struct. Mol. Biol. 13:1135-1140(2006) [PubMed] [Europe PMC] [Abstract]
- "Multiple factors confer specific Cdc42 and Rac protein activation by dedicator of cytokinesis (DOCK) nucleotide exchange factors."J. Biol. Chem. 286:25341-25351(2011) [PubMed] [Europe PMC] [Abstract]