Nitro Tetrazolium Blue (NTB)
Nitro blue tetrazolium is a chemical compound composed of two tetrazole moieties. It is used in immunology for sensitive detection of alkaline phosphatase (with BCIP). NBT serves as the oxidant and BCIP is the AP-substrate (and gives also dark blue dye). In immunohistochemistry the alkaline phosphatase is often used as a marker, conjugated to an antibody. The colored product can either be of the NBT/BCIP reaction reveals where the antibody is bound, or can be used in immunofluorescence. The NBT/BCIP reaction is also used for colorimetric/spectrophotometric activity assays of oxidoreductases. One application is in activity stains in gel electrophoresis, such as with the mitochondrial electron transport chain complexes. Nitro blue tetrazolium is used in a diagnostic test, particularly for chronic granulomatous disease and other diseases of phagocyte function.

Organism species: Pan-species (General)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Complete Antigen of Nitro Tetrazolium Blue (NTB) Antigenic Transformation Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nitro Tetrazolium Blue (NTB) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nitro Tetrazolium Blue (NTB) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nitro Tetrazolium Blue (NTB) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nitro Tetrazolium Blue (NTB) ELISA Kit Customized Service Offer