Nisin (NIS)
Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonate and thioglycolate indicated that the reaction pathway includes a binding step. Reaction of nisin at high pH resulted in the formation of multimeric products, apparently as a result of intramolecular and intermolecular reactions between nucleophilic groups and the dehydro residues. The unique vinyl protons of the dehydro residues that give readily identifiable proton nuclear magnetic resonances were used to observe the addition of nucleophiles to the dehydro moiety. After reaction with nucleophiles, nisin lost its antibiotic activity and no longer showed the dehydro resonances, indicating that the dehydro groups had been modified.

Organism species: Pan-species (General)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Complete Antigen of Nisin (NIS) Antigenic Transformation Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nisin (NIS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nisin (NIS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nisin (NIS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nisin (NIS) ELISA Kit Customized Service Offer