Luciferase U2OS Osteosarcoma Cells (U2OS-Luc)

U2OS-Luc is a cell line derived from the U2OS osteosarcoma cell line that has been genetically modified to express firefly luciferase. Firefly luciferase is an enzyme that catalyzes a reaction with luciferin, resulting in the emission of light. This property allows researchers to measure the activity of the luciferase gene in real-time by detecting the bioluminescent light signal produced. U2OS-Luc cells are commonly used in research studies to study gene expression, promoter activity, drug screening, and other biological processes that involve monitoring changes in gene expression levels.

U-2OS is a common osteosarcoma cell model.  The constructed U-2OS tool cell line could effectively edit protein-coding gene (ETV4) and long non-coding RNA gene (MALAT1). The results of this study also indicated that the constructed U-2OS tool cell line could greatly improve the efficiency of gene editing. Therefore, the genetic engineering cell line provided by the study is of great significance for studying the pathogenesis and regulatory network of osteosarcoma, and for preventing and treating bone tumor as soon as possible.