GP2-293 Embryorenal Epithelial Cells (GP2-293)

GP2-293 cells are a popular cell line used in the production of viral vectors for gene therapy and gene editing applications. These cells are derived from 293 cells, which are human embryonic kidney cells that have been transformed with the SV40 large T antigen for immortalization. GP2-293 cells are specifically engineered to stably express the functional Epstein-Barr virus nuclear antigen 1 (EBNA1) protein, which allows for the episomal replication of vectors containing the Epstein-Barr virus (EBV) origin of replication.

The expression of EBNA1 in GP2-293 cells enables the maintenance and stable replication of plasmids carrying the EBV origin of replication, such as viral vector plasmids used in gene therapy applications. This feature is advantageous for the production of high-titer recombinant adenovirus, AAV, lentivirus, or other viral vectors that require episomal replication.

GP2-293 cells are commonly used in research laboratories and biotechnology companies for the generation of viral vectors for a wide range of applications, including gene delivery, gene editing, and protein expression. Their ability to support high-level vector production and their genetic stability make them a valuable tool for researchers and biomanufacturers working in the field of gene therapy and genetic engineering.