HEK 293F

HEK 293F cell line is a human embryonic kidney cell line that was derived from the original HEK 293 cell line, which was generated by transformingThe HEK 293F cell line is a widely used cell line in biomedical research and biotechnology. The "HEK" stands for Human Embryonic Kidney, indicating the origin of the cells, which were derived from the kidney tissue of a human embryo. The "293" designation refers to the cell line being derived from the 293rd experiment in a series conducted by Frank Graham and colleagues who first established the cell line in the early 1970s.

HEK 293F cells are a specific subline of the HEK 293 cell line that has been adapted for suspension culture. These cells are commonly used for the production of recombinant proteins, viral vectors, and other biopharmaceuticals due to their high transfection efficiency and protein expression capacity. The "F" in the name denotes that the cells are able to grow in suspension, making them suitable for large-scale protein production applications in bioreactors.

Moreover, HEK 293F cells are frequently employed in basic research to study various cellular processes, including protein trafficking, signal transduction, gene expression, and cell biology. They are valuable tools for investigating cellular functions and molecular mechanisms underlying disease states, drug discovery, and therapeutic protein development.

Overall, the HEK 293F cell line has become a prominent workhorse in biotechnology and biomedical research due to its ease of culture, high transfection efficiency, and versatility in applications ranging from basic cell biology to biopharmaceutical production.