M-NFS-60 Myelogenous Leukemia Cells (M-NFS-60)
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Human granulocyte colony-stimulating factor (GCSF) is a well-known cytokine for neutropenia treatment. However, daily injections are required due to the short circulating half-life of the protein. To overcome this bottleneck, we fused GCSF with the Fc domain of IgG1 at the C terminus (GCSF-Fc) and with the maltose binding protein (MBP) tag at the N-terminus and expressed it as a soluble protein in the cytoplasm of E. coli. We also conjugated PEG aldehyde to GCSF to make PEG-GCSF. The bioactivities of GCSF-Fc and PEG-GCSF were similar to native GCSF using the mouse M-NFS-60 myelogenous leukemia cell line. The EC50 dose-response curves for GCSF, GCSF-Fc and PEG-GCSF were 37 ± 12 pM, 75 ± 13.5 pM and 46 ± 5.5 pM, respectively. When the proteins were injected into neutropenic rats, the group injected with PEG-GCSF showed the highest and fastest recovery of neutrophils, followed by GCSF-Fc and GCSF. ELISA assay revealed the PEG-GCSF had the longest plasma circulation (>72 h), followed by GCSF-Fc (>48 h) and GCSF (~24 h), which is consistent with the in vivo activities of the proteins. In summary, the GCSF-Fc purified from E. coli was not as efficient as PEG-GCSF in treating neutropenic rats.
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Cells | CSI371Mu11 | Mouse M-NFS-60 Myelogenous Leukemia Cells (M-NFS-60) | For research use only. It is not approved for human or animal use, or for applications in in vitro diganostic procedures. |
Extracts | n/a | Extract of M-NFS-60 Myelogenous Leukemia Cells (M-NFS-60) | Total Protein/DNA/RNA Extract Customized Service Offer |
Mediums | MSI371Mu11 | Medium for Mouse M-NFS-60 Myelogenous Leukemia Cells (M-NFS-60) | Complete Medium for the culture of M-NFS-60 cell line |