Taste Receptor Type 1 Member 2 (TAS1R2)

Human, rat, and mouse T1Rs are only 70% identical. The authors presented a detailed analysis of the patterns of expression of T1rs and T2rs, thus providing a view of the representation of sweet and bitter taste at the periphery. Lactisole inhibited the activity of the human T1R1/T1R3 receptor and, as predicted, blocked the umami taste of L-glutamate in human taste tests. Cyclamate did not activate the T1R1/T1R3 receptor by itself, but potentiated the receptor's response to L-glutamate. Taken together, these findings demonstrated the different functional roles of T1R3 and T1R2 and the presence of multiple ligand binding sites on the sweet taste receptor.In human NCI-H716 L cells, GLP1 release was promoted by sugars and sucralose and blocked by the sweet-receptor antagonist lactisole or siRNA for alpha-gustducin.