Trafficking Protein, Kinesin Binding 2 (TRAK2)

ALS2CR3 contains a coiled-coil domain that shares significant homology with the coiled-coil region in huntingtin-associated protein-1 (HAP1). Northern blot analysis revealed ubiquitous expression of 7.0- and 4.5-kb transcripts, reflecting the use of 2 alternate polyadenylation signals; expression was highest in heart.

Northern blot analysis detected a major 6.2-kb transcript in all tissues tested except testis, which expressed a truncated transcript of 2.9 kb; a minor 4.2-kb transcript was also detected. Western blot analysis revealed tissue-specific expression of 3 Grif1 species with different molecular masses in brain, heart, and skeletal muscle. Grif1 was expressed in HEK293 cells as 3 proteins with apparent molecular masses of 98, 106, and 115 kD.