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Topoisomerase II Binding Protein 1 (TOPBP1)

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TOP2BP1; DNA topoisomerase II-beta-binding protein 1

Topoisomerase II Binding Protein 1 (TOPBP1)
CCC D801
HGNC 17008
MGI 1920018
PubMed 24239288
RGD 1562949
UniProt Q92547
Wiki TOPBP1
TOPBP1 is a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.The deduced 1,522-amino acid protein has a calculated molecular mass of about 171 kD. TOPBP1 contains 8 repeating regions throughout its sequence that share similarity with repeat regions 1 and 2 of Rad4. It also has an auto-ADP-ribosylation site and 2 C-terminal nuclear localization signals. The 3-prime untranslated region contains 12 AUUUA mRNA-instability motifs. Northern blot analysis detected ubiquitous expression, with smearing characteristic of unstable mRNA. Transcripts above 10 kb were detected only in heart.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD801Hu01 Recombinant Topoisomerase II Binding Protein 1 (TOPBP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD801Hu01 Polyclonal Antibody to Topoisomerase II Binding Protein 1 (TOPBP1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Topoisomerase II Binding Protein 1 (TOPBP1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Topoisomerase II Binding Protein 1 (TOPBP1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD801Mu01 Recombinant Topoisomerase II Binding Protein 1 (TOPBP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD801Mu01 Polyclonal Antibody to Topoisomerase II Binding Protein 1 (TOPBP1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Topoisomerase II Binding Protein 1 (TOPBP1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Topoisomerase II Binding Protein 1 (TOPBP1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD801Ra01 Recombinant Topoisomerase II Binding Protein 1 (TOPBP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD801Ra01 Polyclonal Antibody to Topoisomerase II Binding Protein 1 (TOPBP1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Topoisomerase II Binding Protein 1 (TOPBP1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Topoisomerase II Binding Protein 1 (TOPBP1) ELISA Kit Customized Service Offer
  1. "A DNA topoisomerase II binding protein with eight repeating regions similar to DNA repair enzymes and to a cell cycle regulator."Eur. J. Biochem. 250:794-799(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence, annotation and analysis of human chromosome 3." Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
  5. "Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA."Oncogene 18:5194-5203(1999) [PubMed] [Europe PMC] [Abstract]
  6. "BRCT domain-containing protein TopBP1 functions in DNA replication and damage response."J. Biol. Chem. 276:30399-30406(2001) [PubMed] [Europe PMC] [Abstract]
  7. "Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response."J. Biol. Chem. 277:3599-3605(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Regulation of E2F1 by BRCT domain-containing protein TopBP1."Mol. Cell. Biol. 23:3287-3304(2003) [PubMed] [Europe PMC] [Abstract]
  9. "PML colocalizes with and stabilizes the DNA damage response protein TopBP1."Mol. Cell. Biol. 23:4247-4256(2003) [PubMed] [Europe PMC] [Abstract]
  10. "TopBP1 localises to centrosomes in mitosis and to chromosome cores in meiosis."Chromosoma 112:323-330(2004) [PubMed] [Europe PMC] [Abstract]
  11. "TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival."Genes Dev. 18:673-686(2004) [PubMed] [Europe PMC] [Abstract]
  12. "TopBP1 activates the ATR-ATRIP complex."Cell 124:943-955(2006) [PubMed] [Europe PMC] [Abstract]
  13. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  14. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  15. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  17. "A vertebrate gene, ticrr, is an essential checkpoint and replication regulator."Genes Dev. 24:183-194(2010) [PubMed] [Europe PMC] [Abstract]
  18. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  19. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  20. "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling."Science 332:1313-1317(2011) [PubMed] [Europe PMC] [Abstract]
  21. "The third BRCA1 C-terminus (BRCT) domain of topoisomerase II binding protein."Submitted (NOV-2004) to the PDB data bank
  22. "Insights from the crystal structure of the sixth BRCT domain of topoisomerase IIbeta binding protein 1."Protein Sci. 19:162-167(2010) [PubMed] [Europe PMC] [Abstract]