Procollagen II (PCII)

Type II procollagen is synthesized in two forms (IIA, IIB), generated by the alternative splicing of its precursor mRNA. The recombinant procollagen II had the expected amino acid sequence as defined by nucleotide sequencing of mRNA-derived cDNA and the expected amino acid composition as defined by analysis of Type II Procollagen that was converted into collagen II by digestion with procollagen N- and C-proteinases.

In addition, the protein was shown to have a native conformation as assayed by a series of protease digestions. No essential differences were found between clones transfected with the COL2A1 gene construct containing the COL1A1 promoter and the similar construct containing the COL2A1 promoter in terms of number of clones synthesizing recombinant procollagen II and the levels of expression.