≡

Alanine Aminopeptidase (AAP)

[Edit]

CD13; ALAP; P150; ANPEP; APN; LAP1; PEPN; Gp150; Aminopeptidase M; Microsomal Aminopeptidase; Alanyl Membrane Aminopeptidase; Aminopeptidase N

Alanine Aminopeptidase (AAP)
CCC B065
HGNC 500
MGI 96749
PubMed 15166647
RGD 2991
UniProt P15144
Wiki AAP

Alanine aminopeptidase (EC 3.4.11.2) is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is found at high levels in the urine when there are kidney problems.Also called AAP. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB065Hu01 Recombinant Alanine Aminopeptidase (AAP) Positive Control; Immunogen; SDS-PAGE; WB.
RPB065Hu02 Recombinant Alanine Aminopeptidase (AAP) Positive Control; Immunogen; SDS-PAGE; WB.
EPB065Hu61 Eukaryotic Alanine Aminopeptidase (AAP) Positive Control; Immunogen; SDS-PAGE; WB.
APB065Hu61 Active Alanine Aminopeptidase (AAP) Cell culture; Activity Assays.
Antibodies PAB065Hu01 Polyclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
MAB065Hu22 Monoclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
MAB065Hu26 Monoclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
MAB065Hu21 Monoclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
Assay Kits SEB065Hu ELISA Kit for Alanine Aminopeptidase (AAP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB065Mu01 Recombinant Alanine Aminopeptidase (AAP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB065Mu01 Polyclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Alanine Aminopeptidase (AAP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Alanine Aminopeptidase (AAP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB065Ra01 Recombinant Alanine Aminopeptidase (AAP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB065Ra01 Polyclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
MAB065Ra21 Monoclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IP.
LAB065Ra81 FITC-Linked Polyclonal Antibody to Alanine Aminopeptidase (AAP) WB; IHC; ICC; IF.
Assay Kits SEB065Ra ELISA Kit for Alanine Aminopeptidase (AAP) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Complete amino acid sequence of human intestinal aminopeptidase N as deduced from cloned cDNA."FEBS Lett. 238:307-314(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N."J. Clin. Invest. 83:1299-1307(1989) [PubMed] [Europe PMC] [Abstract]
  3. "Analysis of the DNA sequence and duplication history of human chromosome 15." Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Separate promoters control transcription of the human aminopeptidase N gene in myeloid and intestinal epithelial cells."J. Biol. Chem. 266:11999-12007(1991) [PubMed] [Europe PMC] [Abstract]
  6. "Identification of an alanine aminopeptidase in human maternal serum as a membrane-bound aminopeptidase N."Biol. Chem. Hoppe-Seyler 376:397-400(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Cholesterol crystallization-promoting activity of aminopeptidase-N isolated from the vesicular carrier of biliary lipids."FEBS Lett. 329:84-88(1993) [PubMed] [Europe PMC] [Abstract]
  8. "Purification and characterization of aminopeptidase N from human plasma."Enzyme 32:65-75(1984) [PubMed] [Europe PMC] [Abstract]
  9. "Variable O-glycosylation of CD13 (aminopeptidase N)."J. Biol. Chem. 266:4593-4597(1991) [PubMed] [Europe PMC] [Abstract]
  10. "Human aminopeptidase N is a receptor for human coronavirus 229E."Nature 357:420-422(1992) [PubMed] [Europe PMC] [Abstract]
  11. "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated."Exp. Hematol. 21:1695-1701(1993) [PubMed] [Europe PMC] [Abstract]
  12. "CD13 (human aminopeptidase N) mediates human cytomegalovirus infection."J. Virol. 67:6576-6585(1993) [PubMed] [Europe PMC] [Abstract]
  13. "Characterization of functional domains in the human coronavirus HCV 229E receptor."J. Gen. Virol. 77:2515-2521(1996) [PubMed] [Europe PMC] [Abstract]
  14. "Defectively N-glycosylated and non-O-glycosylated aminopeptidase N (CD13) is normally expressed at the cell surface and has full enzymatic activity."Exp. Cell Res. 231:112-118(1997) [PubMed] [Europe PMC] [Abstract]
  15. "Identification of residues critical for the human coronavirus 229E receptor function of human aminopeptidase N."J. Gen. Virol. 78:2795-2802(1997) [PubMed] [Europe PMC] [Abstract]
  16. "Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N."J. Gen. Virol. 79:1387-1391(1998) [PubMed] [Europe PMC] [Abstract]
  17. "Modification of the amino terminus of a class II epitope confers resistance to degradation by CD13 on dendritic cells and enhances presentation to T cells."J. Immunol. 164:129-135(2000) [PubMed] [Europe PMC] [Abstract]
  18. "Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis."Cancer Res. 60:722-727(2000) [PubMed] [Europe PMC] [Abstract]
  19. "Expression of aminopeptidase N in human endometrium and regulation of its activity by estrogen."Fertil. Steril. 75:1172-1176(2001) [PubMed] [Europe PMC] [Abstract]
  20. "Molecular determinants of species specificity in the coronavirus receptor aminopeptidase N (CD13): influence of N-linked glycosylation."J. Virol. 75:9741-9752(2001) [PubMed] [Europe PMC] [Abstract]
  21. "Soluble aminopeptidase N/CD13 in malignant and nonmalignant effusions and intratumoral fluid."Clin. Cancer Res. 8:3747-3754(2002) [PubMed] [Europe PMC] [Abstract]
  22. "Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E."J. Virol. 77:2530-2538(2003) [PubMed] [Europe PMC] [Abstract]
  23. "A proteomic analysis of human bile."Mol. Cell. Proteomics 3:715-728(2004) [PubMed] [Europe PMC] [Abstract]
  24. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
  25. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  27. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  28. "The X-ray crystal structure of human aminopeptidase N reveals a novel dimer and the basis for peptide processing."J. Biol. Chem. 287:36804-36813(2012) [PubMed] [Europe PMC] [Abstract]
  29. "Identification of point mutations in the aminopeptidase N gene by SSCP analysis and sequencing."Hum. Mutat. Suppl. 1:S158-S160(1998) [PubMed] [Europe PMC] [Abstract]