![Hepatoma Derived Growth Factor (HDGF)](/upload/item/img/Hepatoma-Derived-Growth-Factor-(HDGF)-90624.jpg)
Nakamura et al. (1994) purified a novel hepatoma-derived growth factor from the conditioned medium of human hepatoma-derived cell line HuH-7. Molecular cloning of a cDNA from the cDNA library of the same cell line was done on the basis of the N-terminal amino acid sequence. The cDNA was 2.4 kb long and the deduced amino acid sequence contained 240 amino acids without a signal peptide-like N-terminal hydrophobic sequence. The primary sequence shared homology with the high mobility group-1 protein; they showed 23.4% amino acid identity and 35.6% amino acid similarity.By immunofluorescence study, Nakamura et al. (1994) showed that HDGF is localized in the cytoplasm of hepatoma cells and northern blots showed that it is expressed ubiquitously in normal tissues and tumor cell lines.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA624Hu01 | Recombinant Hepatoma Derived Growth Factor (HDGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAA624Hu01 | Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF) | WB; IHC; ICC; IP; FCM |
MAA624Hu22 | Monoclonal Antibody to Hepatoma Derived Growth Factor (HDGF) | WB; IHC; ICC; IP. | |
Assay Kits | SEA624Hu | ELISA Kit for Hepatoma Derived Growth Factor (HDGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA624Mu01 | Recombinant Hepatoma Derived Growth Factor (HDGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA624Mu02 | Recombinant Hepatoma Derived Growth Factor (HDGF) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA624Mu01 | Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF) | WB; IHC; ICC; IP. |
PAA624Mu02 | Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF) | WB; IHC; ICC; IP. | |
Assay Kits | SEA624Mu | ELISA Kit for Hepatoma Derived Growth Factor (HDGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA624Ra01 | Recombinant Hepatoma Derived Growth Factor (HDGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAA624Ra01 | Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF) | WB; IHC; ICC; IP. |
Assay Kits | n/a | CLIA Kit for Hepatoma Derived Growth Factor (HDGF) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Hepatoma Derived Growth Factor (HDGF) | ELISA Kit Customized Service Offer |
- "Molecular cloning of complementary DNA for a novel human hepatoma-derived growth factor. Its homology with high mobility group-1 protein."J. Biol. Chem. 269:25143-25149(1994) [PubMed] [Europe PMC] [Abstract]
- "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Hepatoma-derived growth factor stimulates cell growth after translocation to the nucleus by nuclear localization signals."J. Biol. Chem. 277:10315-10322(2002) [PubMed] [Europe PMC] [Abstract]
- "Large-scale characterization of HeLa cell nuclear phosphoproteins."Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed] [Europe PMC] [Abstract]
- "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
- "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
- "Phosphoproteomic analysis of the human pituitary."Pituitary 9:109-120(2006) [PubMed] [Europe PMC] [Abstract]
- "Hepatoma-derived growth factor binds DNA through the N-terminal PWWP domain."BMC Mol. Biol. 8:101-101(2007) [PubMed] [Europe PMC] [Abstract]
- "Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
- "SUMOylation of the hepatoma-derived growth factor negatively influences its binding to chromatin."FEBS J. 275:1411-1426(2008) [PubMed] [Europe PMC] [Abstract]
- "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
- "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
- "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
- "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
- "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
- "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
- "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
- "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
- "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
- "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
- "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
- "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
- "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
- "PWWP module of human hepatoma-derived growth factor forms a domain-swapped dimer with much higher affinity for heparin."J. Mol. Biol. 367:456-472(2007) [PubMed] [Europe PMC] [Abstract]
- "Solution structure and heparin interaction of human hepatoma-derived growth factor."J. Mol. Biol. 343:1365-1377(2004) [PubMed] [Europe PMC] [Abstract]