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Damage Specific DNA Binding Protein 2 (DDB2)

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DDBB; UV-DDB2; Xeroderma Pigmentosum Group E Protein; UV-Damaged DNA-Binding Protein 2; DDB p48 Subunit; Damage-specific DNA-binding protein 2

Damage Specific DNA Binding Protein 2 (DDB2)
CCC A319
HGNC 2718
MGI 1355314
PubMed 16260596
UniProt Q92466
Wiki DDB2
DDB2 is the smaller subunit of a heterodimeric protein implicated in the etiology of xeroderma pigmentosum group E. This subunit appears to be required for DNA binding.
This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA319Hu01 Recombinant Damage Specific DNA Binding Protein 2 (DDB2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA319Hu01 Polyclonal Antibody to Damage Specific DNA Binding Protein 2 (DDB2) WB; IHC; ICC; IP.
Assay Kits SEA319Hu ELISA Kit for Damage Specific DNA Binding Protein 2 (DDB2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA319Mu01 Recombinant Damage Specific DNA Binding Protein 2 (DDB2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA319Mu01 Polyclonal Antibody to Damage Specific DNA Binding Protein 2 (DDB2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Damage Specific DNA Binding Protein 2 (DDB2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Damage Specific DNA Binding Protein 2 (DDB2) ELISA Kit Customized Service Offer
  1. "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein."Genomics 29:62-69(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair."Biochem. Biophys. Res. Commun. 314:1036-1043(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "p48 Activates a UV-damaged-DNA binding factor and is defective in xeroderma pigmentosum group E cells that lack binding activity."Mol. Cell. Biol. 18:4391-4399(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Expression of the p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair."Proc. Natl. Acad. Sci. U.S.A. 96:424-428(1999) [PubMed] [Europe PMC] [Abstract]
  7. "Human damage-specific DNA-binding protein p48. Characterization of XPE mutations and regulation following UV irradiation."J. Biol. Chem. 275:21422-21428(2000) [PubMed] [Europe PMC] [Abstract]
  8. "Nuclear transport of human DDB protein induced by ultraviolet light."J. Biol. Chem. 275:21429-21434(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Xeroderma pigmentosum p48 gene enhances global genomic repair and suppresses UV-induced mutagenesis."Mol. Cell 5:737-744(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A."J. Biol. Chem. 276:15434-15440(2001) [PubMed] [Europe PMC] [Abstract]
  11. "UV-damaged DNA-binding proteins are targets of CUL-4A-mediated ubiquitination and degradation."J. Biol. Chem. 276:48175-48182(2001) [PubMed] [Europe PMC] [Abstract]
  12. "DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair."J. Biol. Chem. 277:1637-1640(2002) [PubMed] [Europe PMC] [Abstract]
  13. "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage."Cell 113:357-367(2003) [PubMed] [Europe PMC] [Abstract]
  14. "In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product."J. Biol. Chem. 278:46906-46910(2003) [PubMed] [Europe PMC] [Abstract]
  15. "UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex."Cell 121:387-400(2005) [PubMed] [Europe PMC] [Abstract]
  16. "DDB1-DDB2 (xeroderma pigmentosum group E) protein complex recognizes a cyclobutane pyrimidine dimer, mismatches, apurinic/apyrimidinic sites, and compound lesions in DNA."J. Biol. Chem. 280:39982-39989(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Xeroderma pigmentosum complementation group E protein (XPE/DDB2): purification of various complexes of XPE and analyses of their damaged DNA binding and putative DNA repair properties."Mol. Cell. Biol. 25:9784-9792(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  19. "DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases."Genes Dev. 20:2949-2954(2006) [PubMed] [Europe PMC] [Abstract]
  20. "Cullin 4A-mediated proteolysis of DDB2 protein at DNA damage sites regulates in vivo lesion recognition by XPC."J. Biol. Chem. 281:13404-13411(2006) [PubMed] [Europe PMC] [Abstract]
  21. "Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage."Mol. Cell 22:383-394(2006) [PubMed] [Europe PMC] [Abstract]
  22. "A kinase-independent function of c-Abl in promoting proteolytic destruction of damaged DNA binding proteins."Mol. Cell 22:489-499(2006) [PubMed] [Europe PMC] [Abstract]
  23. "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1."Mol. Cell 23:709-721(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery."Nature 443:590-593(2006) [PubMed] [Europe PMC] [Abstract]
  25. "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation."Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract]
  26. "The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites."Proc. Natl. Acad. Sci. U.S.A. 103:2588-2593(2006) [PubMed] [Europe PMC] [Abstract]
  27. "Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC."J. Cell Sci. 120:2706-2716(2007) [PubMed] [Europe PMC] [Abstract]
  28. "The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A."Cancer Res. 68:5014-5022(2008) [PubMed] [Europe PMC] [Abstract]
  29. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  30. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  32. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  33. "The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability."Cell Cycle 11:4378-4384(2012) [PubMed] [Europe PMC] [Abstract]
  34. "Structural basis of UV DNA-damage recognition by the DDB1-DDB2 complex."Cell 135:1213-1223(2008) [PubMed] [Europe PMC] [Abstract]
  35. "A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery."Nat. Struct. Mol. Biol. 17:105-111(2010) [PubMed] [Europe PMC] [Abstract]
  36. "Damaged DNA induced UV-damaged DNA-binding protein (UV-DDB) dimerization and its roles in chromatinized DNA repair."Proc. Natl. Acad. Sci. U.S.A. 109:E2737-E2746(2012) [PubMed] [Europe PMC] [Abstract]
  37. "Mutations specific to the xeroderma pigmentosum group E Ddb-phenotype."J. Biol. Chem. 271:24317-24320(1996) [PubMed] [Europe PMC] [Abstract]