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Protein Kinase C Eta (PKCh)

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PRKCH; PRKC-H; PKC-L; PRKCL; NPKC-Eta

Protein Kinase C Eta (PKCh)
CCC A233
HGNC 9403
MGI 97600
PubMed 9763653
RGD 621888
UniProt P24723
Wiki PRKCH
By screening a human keratinocyte cDNA library using PRKCA fragments as probes, Bacher et al. (1991) isolated a cDNA encoding PRKCH, which they called PKCL. The predicted PRKCH protein contains 680 amino acids and shows highest homology with PRKCE. PRKCH has a consensus ATP-binding site, a catalytic kinase domain, and a conserved cysteine-rich domain. Northern blot analysis of rat tissues detected a 4.2-kb transcript at highest levels in lung, with lower levels in heart and skin. The PRKCH transcript was also detected in the human skin carcinoma cell line SCL-1, the human epidermoid carcinoma cell line A431, and in HaCaT human skin keratinocytes. Transfection of COS cells with a PRKCH expression vector determined that this protein has phorbol ester-binding ability and kinase activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA233Hu01 Recombinant Protein Kinase C Eta (PKCh) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA233Hu01 Polyclonal Antibody to Protein Kinase C Eta (PKCh) WB; ICC; IHC-P; IHC-F; ELISA.
MAA233Hu22 Monoclonal Antibody to Protein Kinase C Eta (PKCh) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Kinase C Eta (PKCh) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Eta (PKCh) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA233Mu01 Recombinant Protein Kinase C Eta (PKCh) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA233Mu01 Polyclonal Antibody to Protein Kinase C Eta (PKCh) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Kinase C Eta (PKCh) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Eta (PKCh) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA233Ra01 Recombinant Protein Kinase C Eta (PKCh) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA233Ra01 Polyclonal Antibody to Protein Kinase C Eta (PKCh) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Kinase C Eta (PKCh) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Eta (PKCh) ELISA Kit Customized Service Offer

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA233Bo01 Recombinant Protein Kinase C Eta (PKCh) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA233Bo01 Polyclonal Antibody to Protein Kinase C Eta (PKCh) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Kinase C Eta (PKCh) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Eta (PKCh) ELISA Kit Customized Service Offer
  1. "Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart."Mol. Cell. Biol. 11:126-133(1991) [PubMed] [Europe PMC] [Abstract]
  2. ErratumMol. Cell. Biol. 12:1404-1404(1992) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Identification of multiple, novel, protein kinase C-related gene products."FEBS Lett. 356:5-8(1994) [PubMed] [Europe PMC] [Abstract]
  7. "Overexpression of protein kinase C-eta attenuates caspase activation and tumor necrosis factor-alpha-induced cell death."Biochem. Biophys. Res. Commun. 279:103-107(2000) [PubMed] [Europe PMC] [Abstract]
  8. "Phorbol 12-myristate 13-acetate induces protein kinase ceta-specific proliferative response in astrocytic tumor cells."J. Biol. Chem. 275:22348-22354(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Protein kinase C-eta regulates resistance to UV- and gamma-irradiation-induced apoptosis in glioblastoma cells by preventing caspase-9 activation."Neuro-oncol. 4:9-21(2002) [PubMed] [Europe PMC] [Abstract]
  10. "PKC-eta mediates glioblastoma cell proliferation through the Akt and mTOR signaling pathways."Oncogene 23:9062-9069(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Translocation of diacylglycerol kinase theta from cytosol to plasma membrane in response to activation of G protein-coupled receptors and protein kinase C."J. Biol. Chem. 280:9870-9878(2005) [PubMed] [Europe PMC] [Abstract]
  12. "The protein kinase C-eta isoform induces proliferation in glioblastoma cell lines through an ERK/Elk-1 pathway."Oncogene 26:2885-2893(2007) [PubMed] [Europe PMC] [Abstract]
  13. "PKC eta directs induction of IRF-4 expression and Ig kappa gene rearrangement in pre-BCR signaling pathway."Int. Immunol. 20:1417-1426(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  15. "PKC eta regulates occludin phosphorylation and epithelial tight junction integrity."Proc. Natl. Acad. Sci. U.S.A. 106:61-66(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Protein kinase C-eta and phospholipase D2 pathway regulates foam cell formation via regulator of G protein signaling 2."Mol. Pharmacol. 78:478-485(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Protein kinase C? activates NF-?B in response to camptothecin-induced DNA damage."Biochem. Biophys. Res. Commun. 412:313-317(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Protein kinase C eta (PKC eta): its involvement in keratinocyte differentiation."J. Biochem. 132:853-857(2002) [PubMed] [Europe PMC] [Abstract]
  19. "PKCepsilon promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria."Cell 148:543-555(2012) [PubMed] [Europe PMC] [Abstract]
  20. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  21. "Structure of human protein kinase C eta (PKCeta) C2 domain and identification of phosphorylation sites."Biochem. Biophys. Res. Commun. 349:1182-1189(2006) [PubMed] [Europe PMC] [Abstract]
  22. "A nonsynonymous SNP in PRKCH (protein kinase C eta) increases the risk of cerebral infarction."Nat. Genet. 39:212-217(2007) [PubMed] [Europe PMC] [Abstract]
  23. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]