Tumor Necrosis Factor Alpha (TNFa)
[Edit]DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Organism species: Homo sapiens (Human)
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Mu01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA133Mu02 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. | |
EPA133Mu61 | Eukaryotic Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. | |
APA133Mu01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
APA133Mu61 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
EPA133Mu62 | Eukaryotic Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA133Mu01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
LAA133Mu71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. | |
PAA133Mu02 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
MAA133Mu21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
PAA133Mu06 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SCA133Mu | CLIA Kit for Tumor Necrosis Factor Alpha (TNFa) | Chemiluminescent immunoassay for Antigen Detection. |
SEA133Mi | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. | |
SEA133Mu | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. | |
HEA133Mu | High Sensitive ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. | |
IEA133Mu | Instant ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Rattus norvegicus (Rat)
Organism species: Cavia (Guinea pig )
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Gu01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Gu01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Gu01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
MAA133Gu21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Gu | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Oryctolagus cuniculus (Rabbit)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Rb01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA133Rb02 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. | |
APA133Rb01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
APA133Rb02 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Rb52 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
PAA133Rb51 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
LAA133Rb71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. | |
MAA133Rb21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
PAA133Rb01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Rb | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Rhesus monkey (Simian)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Si01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Si01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Si01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
LAA133Si71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. | |
LAA133Si81 | FITC-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IF. | |
MAA133Si21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Si | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Canis familiaris; Canine (Dog)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Ca01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Ca01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Ca01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
LAA133Ca71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. | |
LAA133Ca81 | FITC-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IF. | |
MAA133Ca21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Ca | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Sus scrofa; Porcine (Pig)
Organism species: Bos taurus; Bovine (Cattle)
Organism species: Capra hircus; Caprine (Goat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Cp01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA133Cp61 | Eukaryotic Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA133Cp01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
MAA133Cp21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Cp | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Ovis aries; Ovine (Sheep)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Ov01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Ov01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Ov01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
LAA133Ov71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. | |
MAA133Ov21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Ov | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Equus caballus; Equine (Horse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Eq01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Eq01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Eq01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
MAA133Eq21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. | |
Assay Kits | SEA133Eq | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Chicken (Gallus)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Ga01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Ga01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | PAA133Ga01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
Assay Kits | SEA133Ga | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Danio rerio (Zebrafish)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA133Fi01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA133Fi01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. | |
Antibodies | n/a | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Tumor Necrosis Factor Alpha (TNFa) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | ELISA Kit Customized Service Offer |
- "Tandem arrangement of genes coding for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome."Cold Spring Harb. Symp. Quant. Biol. 51:611-624(1986) [PubMed] [Europe PMC] [Abstract]
- "Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin."Nature 312:724-729(1984) [PubMed] [Europe PMC] [Abstract]
- "Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor."Nature 313:803-806(1985) [PubMed] [Europe PMC] [Abstract]
- "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization."Nucleic Acids Res. 13:6361-6373(1985) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning of the complementary DNA for human tumor necrosis factor."Science 228:149-154(1985) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning and expression of human tumor necrosis factor and comparison with mouse tumor necrosis factor."Eur. J. Biochem. 152:515-522(1985) [PubMed] [Europe PMC] [Abstract]
- "Dense Alu clustering and a potential new member of the NF kappa B family within a 90 kilobase HLA class III segment."Nat. Genet. 3:137-145(1993) [PubMed] [Europe PMC] [Abstract]
- "A new member of the Ig superfamily and a V-ATPase G subunit are among the predicted products of novel genes close to the TNF locus in the human MHC."J. Immunol. 162:4745-4754(1999) [PubMed] [Europe PMC] [Abstract]
- "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "O-glycosylated species of natural human tumor-necrosis factor-alpha."Eur. J. Biochem. 235:431-437(1996) [PubMed] [Europe PMC] [Abstract]
- "Phosphorylation of the 26 kDa TNF precursor in monocytic cells and in transfected HeLa cells."J. Inflamm. 45:152-160(1995) [PubMed] [Europe PMC] [Abstract]
- "A casein kinase I motif present in the cytoplasmic domain of members of the tumour necrosis factor ligand family is implicated in 'reverse signalling'."EMBO J. 18:2119-2126(1999) [PubMed] [Europe PMC] [Abstract]
- "Localization of the active site of human tumour necrosis factor (hTNF) by mutational analysis."EMBO J. 10:827-836(1991) [PubMed] [Europe PMC] [Abstract]
- "Myristyl acylation of the tumor necrosis factor alpha precursor on specific lysine residues."J. Exp. Med. 176:1053-1062(1992) [PubMed] [Europe PMC] [Abstract]
- "Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha." Nature 385:733-736(1997) [PubMed] [Europe PMC] [Abstract]
- "A polymorphism that affects OCT-1 binding to the TNF promoter region is associated with severe malaria."Nat. Genet. 22:145-150(1999) [PubMed] [Europe PMC] [Abstract]
- "SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production."Nat. Cell Biol. 8:843-848(2006) [PubMed] [Europe PMC] [Abstract]
- "A gamma-secretase-like intramembrane cleavage of TNFalpha by the GxGD aspartyl protease SPPL2b."Nat. Cell Biol. 8:894-896(2006) [PubMed] [Europe PMC] [Abstract]
- "Phosphorylation of FOXP3 controls regulatory T cell function and is inhibited by TNF-alpha in rheumatoid arthritis."Nat. Med. 19:322-328(2013) [PubMed] [Europe PMC] [Abstract]
- "Structure of tumour necrosis factor."Nature 338:225-228(1989) [PubMed] [Europe PMC] [Abstract]
- "The structure of tumour necrosis factor -- implications for biological function."J. Cell Sci. Suppl. 13:11-18(1990) [PubMed] [Europe PMC] [Abstract]
- "The structure of tumor necrosis factor-alpha at 2.6-A resolution. Implications for receptor binding."J. Biol. Chem. 264:17595-17605(1989) [PubMed] [Europe PMC] [Abstract]
- "Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2."Protein Eng. 10:1101-1107(1997) [PubMed] [Europe PMC] [Abstract]
- "High resolution crystal structure of a human tumor necrosis factor-alpha mutant with low systemic toxicity."J. Biol. Chem. 273:2153-2160(1998) [PubMed] [Europe PMC] [Abstract]
- "Cytokine gene polymorphisms: association with psoriatic arthritis susceptibility and severity."Arthritis Rheum. 48:1408-1413(2003) [PubMed] [Europe PMC] [Abstract]
- "Association of TNF-alpha promoter polymorphisms with the clearance of hepatitis B virus infection."Hum. Mol. Genet. 12:2541-2546(2003) [PubMed] [Europe PMC] [Abstract]