Wide-range ELISA Kit for Casein Beta (CSN2)



This assay has high sensitivity and excellent specificity for detection of Wide-range Casein Beta (CSN2).
No significant cross-reactivity or interference between Wide-range Casein Beta (CSN2) and analogues was observed.


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Casein Beta (CSN2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Casein Beta (CSN2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
中国生物化学与分子生物学报 Pten 对奶牛乳腺上皮细胞活力及乳蛋白合成的影响 Bjmu:Source
Biotechnology and Bioprocess Engineering A modified piggybac transposon system mediated by exogenous mRNA to perform gene delivery in bovine mammary epithelial cells Springer:Source
International Journal of Biometeorology Up-reCavia (Guinea pig )lation of milk secretion with modified microclimate through manipulating plasminogen-plasmin systemin Murrah buffaloes during hot dry season. Pubmed:27174413
J Anim Physiol Anim Nutr (Berl). Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season. pubmed:28084661
Animal Science Journal Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells pubmed:28804941
Journal of Dairy Science Inhibition of arginase via jugular infusion of Nω-hydroxy-nor-l-arginine inhibits casein synthesis in lactating dairy cows Pubmed:29397169
Veterinarski Arhiv The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid … 10.24099:vet.arhiv.170113
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