Wide-range ELISA Kit for Casein Beta (CSN2)
- Product No.WEJ332Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeBreast milk and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 33ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Casein Beta (CSN2).
No significant cross-reactivity or interference between Wide-range Casein Beta (CSN2) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Casein Beta (CSN2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Casein Beta (CSN2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|中国生物化学与分子生物学报||Ｐｔｅｎ 对奶牛乳腺上皮细胞活力及乳蛋白合成的影响 Bjmu:Source|
|Biotechnology and Bioprocess Engineering||A modified piggybac transposon system mediated by exogenous mRNA to perform gene delivery in bovine mammary epithelial cells Springer:Source|
|International Journal of Biometeorology||Up-reCavia (Guinea pig )lation of milk secretion with modified microclimate through manipulating plasminogen-plasmin systemin Murrah buffaloes during hot dry season. Pubmed:27174413|
|J Anim Physiol Anim Nutr (Berl).||Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season. pubmed:28084661|
|Animal Science Journal||Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells pubmed:28804941|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPJ332Hu01||Recombinant Casein Beta (CSN2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAJ332Hu01||Polyclonal Antibody to Casein Beta (CSN2)||WB; IHC; ICC; IP.|
|MAJ332Hu21||Monoclonal Antibody to Casein Beta (CSN2)||WB; IHC; ICC; IP.|
|WEJ332Hu||Wide-range ELISA Kit for Casein Beta (CSN2)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEJ332Hu||ELISA Kit for Casein Beta (CSN2)||Enzyme-linked immunosorbent assay for Antigen Detection.|