Wide-range ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)


This assay has high sensitivity and excellent specificity for detection of Wide-range Amyloid Beta Peptide 1-42 (Ab1-42).
No significant cross-reactivity or interference between Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-104 101
EDTA plasma(n=5) 89-96 93
heparin plasma(n=5) 98-105 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-101% 81-94% 79-93% 91-101%
EDTA plasma(n=5) 88-104% 83-103% 85-93% 93-102%
heparin plasma(n=5) 84-91% 97-105% 81-104% 91-101%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Neuropsychopharmacology Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease Pubmed: 23493042
Neuropharmacology and analgesia The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1–42 seeding and short-term memory deficits in the Aβ25–35 mouse model of Alzheimer's disease Pubmed: 23123349
世界科技研究与发展(World Sci-tech R & D) 慢病毒介导的CBS基因过表达对原代皮质神经元 Globesci:Source
南方医科大学学报 外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响 J-Smu:Source
Neurochemical research Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain Pubmed:24792732
Indian J Biochem Biophys. Amyloid ? lowering and cognition enhancing effects of ghrelin receptor analog [D-Lys (3)] GHRP-6 in rat model of obesity Pubmed:25296496
Cell Mol Neurobiol Anesthetic Propofol Attenuates Apoptosis, Aβ Accumulation, and Inflammation Induced by Sevoflurane Through NF-κB Pathway in Human Neuroglioma Cells PubMed: 25809614
Neuropsychiatr Dis Treat Autophagy dysfunction upregulates beta-amyloid peptides via enhancing the activity of γ-secretase complex PubMed: 26316755
Neurochem Int Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS2 transgenic model of Alzheimer&#039;s disease PubMed: 25882166
Journal of Alzheimer’s Parkinsonism & Dementia The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats open-access:
世界科技研究与发展 芝麻素对高糖条件下PC12细胞Aβ和BACE-1表达的影响 article:10299
Am J Alzheimers Dis Other Demen. Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease Pubmed:26993489
世界科技研究与发展 芝麻素对高糖条件下PC12 细胞Aß 和BACE 1 表达的影响 QK:92621A
Archives of Pharmacal Research Geniposide attenuates the level of Aβ1–42 via enhancing leptin signaling in cellular and APP/PS1 transgenic mice pubmed:28160136
Food Funct. Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease. pubmed:28232989
Scientific Reports Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s disease articles:srep41041.ris
Journal of Medicinal Food Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cells pubmed:28406733
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