Wide-range ELISA Kit for Endostatin (ES)
- Product No.WEA542Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.63ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Endostatin (ES).
No significant cross-reactivity or interference between Wide-range Endostatin (ES) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Endostatin (ES) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Endostatin (ES) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Endostatin (ES) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Endostatin (ES) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Endostatin (ES) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Cancer Immunology||Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice SpringerLink: u38777121t6660l5|
|Journal of Fluoresence||Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy SpringerLink: 4v306130302257w0|
|The Journal of Gene Medicine||Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma Wiley: source|
|Biomedicine & Pharmacotherapy||Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma ScienceDirect: S075333221100151X|
|Science Alert||Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source|
|The Journal of Immunology||Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer PubMed: PMC3619527|
|Prostaglandins & Other Lipid Mediators||Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema Pubmed:24911647|
|American Journal of Physiology - Heart and Circulatory Physiology||Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? Source|
|shock||Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome PubMed: 26125086|
|Clin Rev Allergy Immunol||Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066|
|Angiologia e Cirurgia Vascular||Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052|
|Clinical Rheumatology||Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752|
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