Wide-range ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)
CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
- Product No.WEA087Mi
- Organism SpeciesMus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.064ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 616
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For more details, please contact local distributors! US$ 5236
For more details, please contact local distributors! US$ 43120
For more details, please contact local distributors!
This assay has high sensitivity for detection of Wide-range Monocyte Chemotactic Protein 1 (MCP1).
100% cross-reactivity of Wide-range Monocyte Chemotactic Protein 1 (MCP1) was observed among Mouse, Rat.
Matrices listed below were spiked with certain level of recombinant Wide-range Monocyte Chemotactic Protein 1 (MCP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Monocyte Chemotactic Protein 1 (MCP1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Monocyte Chemotactic Protein 1 (MCP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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|Catalog No.||Related products for research use of Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA087Mi02||Recombinant Monocyte Chemotactic Protein 1 (MCP1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|SEA087Mi||ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|WEA087Mi||Wide-range ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)||Enzyme-linked immunosorbent assay for Antigen Detection.|