≡

ELISA Kit for Suppression Of Tumorigenicity 14 (ST14)

SNC19; HAI; MT-SP1; PRSS14; TADG15; Epithin; Matriptase; Prostamin; Membrane-type serine protease 1; Serine protease 14; Tumor-associated differentially-expressed gene 15

Specificity

This assay has high sensitivity and excellent specificity for detection of Suppression Of Tumorigenicity 14 (ST14).
No significant cross-reactivity or interference between Suppression Of Tumorigenicity 14 (ST14) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppression Of Tumorigenicity 14 (ST14) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppression Of Tumorigenicity 14 (ST14) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPP657Hu01 Recombinant Suppression Of Tumorigenicity 14 (ST14) Positive Control; Immunogen; SDS-PAGE; WB.
PAP657Hu01 Polyclonal Antibody to Suppression Of Tumorigenicity 14 (ST14) WB; IHC; ICC; IP.
LAP657Hu81 FITC-Linked Polyclonal Antibody to Suppression Of Tumorigenicity 14 (ST14) WB; IHC; ICC; IF.
LAP657Hu71 Biotin-Linked Polyclonal Antibody to Suppression Of Tumorigenicity 14 (ST14) WB; IHC; ICC.
MAP657Hu21 Monoclonal Antibody to Suppression Of Tumorigenicity 14 (ST14) WB,IHC
LAP657Hu72 Biotin-Linked Monoclonal Antibody to Suppression Of Tumorigenicity 14 (ST14) WB; IHC; ICC.
SEP657Hu ELISA Kit for Suppression Of Tumorigenicity 14 (ST14) Enzyme-linked immunosorbent assay for Antigen Detection.
LMP657Hu Multiplex Assay Kit for Suppression Of Tumorigenicity 14 (ST14) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSP657Hu01 Antibody Pair for Suppression Of Tumorigenicity 14 (ST14) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSP657Hu01 ELISA Kit DIY Materials for Suppression Of Tumorigenicity 14 (ST14) Main materials for "Do It (ELISA Kit) Yourself".