ELISA Kit for Pentraxin 3, Long (PTX3)
TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein
- Product No.SEK411Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.6pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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For more details, please contact local distributors! US$ 53200
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This assay has high sensitivity and excellent specificity for detection of Pentraxin 3, Long (PTX3).
No significant cross-reactivity or interference between Pentraxin 3, Long (PTX3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Pentraxin 3, Long (PTX3) and the recovery rates were calculated by comparing the measured value to the expected amount of Pentraxin 3, Long (PTX3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pentraxin 3, Long (PTX3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pentraxin 3, Long (PTX3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pentraxin 3, Long (PTX3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Mediators of Inflammation||Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model PubMed: 22966213|
|Ibnosina Journal of Medicine and Biomedical Sciences||Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand Rabbits Sfu: Source|
|Hemodialysis International||Hemodialysis-induced changes in the blood composition affect function of the endothelium Onlinelibrary: hdi.12148|
|Hemodial Int.||Hemodialysis‐induced changes in the blood composition affect function of the endothelium Pubmed:24612443|
|Journal of Obstetrics and Gynaecology Research||The effects of sunitinib on endometriosis Pubmed:25093747|
|Gynecol Endocrinol.||Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic rats Pubmed:25366063|
|Taiwanese Journal of Obstetrics and Gynecology||Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3 Pubmed:25510691|
|Journal of Obstetrics and Gynaecology Research||Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parameters PubMed: 26223286|
|J Obstet Gynaecol||The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat model PubMed: 26470829|
|Neurology Research International||Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers 5952149|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPK411Ra01||Recombinant Pentraxin 3, Long (PTX3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAK411Ra01||Polyclonal Antibody to Pentraxin 3, Long (PTX3)||WB; IHC; ICC; IP.|
|MAK411Ra21||Monoclonal Antibody to Pentraxin 3, Long (PTX3)||WB; IHC; ICC; IP.|
|SEK411Ra||ELISA Kit for Pentraxin 3, Long (PTX3)||Enzyme-linked immunosorbent assay for Antigen Detection.|