ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)

ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle

Specificity

This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2).
No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
The Journal of Heart and Lung Transplantation SERCA2a: A potential non-invasive biomarker of cardiac allograft rejection. pubmed:28750934
researchsquare Value of SERCA2a as a Biomarker for the Identification of Patients With Advanced Heart Failure Requiring Circulatory Support
Journal of Personalized Medicine Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support 34834474
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPG374Hu01 Recombinant ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Positive Control; Immunogen; SDS-PAGE; WB.
RPG374Hu02 Recombinant ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Positive Control; Immunogen; SDS-PAGE; WB.
PAG374Hu01 Polyclonal Antibody to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) WB; IHC; ICC; IP.
PAG374Hu02 Polyclonal Antibody to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) WB; IHC; ICC; IP.
MAG374Hu21 Monoclonal Antibody to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) WB; IHC; ICC; IP.
SEG374Hu ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG374Hu Multiplex Assay Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSG374Hu01 ELISA Kit DIY Materials for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Main materials for "Do It(ELISA Kit) Yourself"